The detection and subsequent volume optimization of biological nanocrystals

Structural Dynamics
Joseph R LuftGaetano T Montelione

Abstract

Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.

References

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Citations

Mar 6, 2016·Archives of Biochemistry and Biophysics·Christopher O BarnesGuillermo Calero
Dec 17, 2016·Journal of Applied Crystallography·Andrew E BrunoEdward H Snell
Dec 5, 2019·Journal of Applied Crystallography·John H BealeRobin L Owen

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Methods Mentioned

BETA
X-ray
light-scattering
electron microscopy
Fluorescence
imaging technique

Software Mentioned

AGF
HTSlab
CLANS
Formulatrix
MALS

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