PMID: 38312Jul 1, 1979

The detection of enzyme induction by rat liver microsomes prepared by isoelectric precipitation

The Journal of Pharmacy and Pharmacology
A Parkinson, S Safe

Abstract

A comparison was made of various indices of the hepatic drug-metabolizing apparatus associated with the postmitochondrial superntant, microsomes harvested by differential centrifugation, and microsomes harvested by isoelectric precipitation from control, phenobarbitone-pretreated and 3-methylcholanthrene-pretreated rats. The metabolic capabilities and distinctions between control and induced rats for each of the three liver preparations compared favourably as determined by the concentration of cytochrome b5 and P-450 and by the activity of NADPH-cytochrome c reductase, 4-dimethylaminoantipyrine N-demethylase and aryl hydrocarbon (benzo[a]pyrene) hydroxylase. The results suggest that the relatively simple isoelectric precipitation technique is a useful alternative to the conventional method of differential centrifugation for the preparation of hepatic microsomes.

References

Mar 1, 1989·Biochemical Pharmacology·A S KosterA C Frankhuijzen-Sierevogel
Jan 1, 1986·Toxicology Letters·K RozmanA Parkinson
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·F E Burley, T M Bray
Jun 1, 1980·Chemico-biological Interactions·A ParkinsonS Safe
Feb 1, 1981·Toxicology and Applied Pharmacology·L RobertsonS Safe
Jan 1, 1990·Journal of Biochemical Toxicology·L van BreeJ de Vries
Jan 1, 1988·Journal of Biochemical Toxicology·D R DuttonA Parkinson

Citations

May 15, 1977·Analytical Biochemistry·S NesnowC R Jefcoate
Feb 1, 1975·Analytical Biochemistry·J W DePierreL Ernster
Sep 1, 1968·Biochemical Pharmacology·J H Dewaide, P T Henderson
Sep 18, 1972·European Journal of Biochemistry·C A BlythB R Rabin
Aug 16, 1974·Science·D M Jerina, J W Daly
Jul 1, 1970·Biochemical Pharmacology·T C Pederson, S D Aust
Sep 8, 1966·Biochemical and Biophysical Research Communications·N E Sladek, G J Mannering
Nov 30, 1967·Biochemical and Biophysical Research Communications·A P AlvaresR Kuntzman

Related Concepts

Centrifugation, Density Gradient
NADPH-Ferrihemoprotein Reductase
Methylcholanthrene
Benzopyrene Hydroxylase
Cytochrome P-450 Oxygenase
Microsomes, Liver
Cytochromes
Oxidoreductases, N-Demethylating
Enzyme Induction
Luminal

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