The Detection of Genetic Parameters for Prognostic Stratification of Neuroblastoma Using Multiplex Ligation-Dependent Probe Amplification Technique

Genetic Testing and Molecular Biomarkers
Sait TumerH Nur Olgun

Abstract

Neuroblastoma (NB) is a neoplasm of the sympathetic nervous system and the most frequent extra cranial solid tumor of early childhood. These tumors display a wide range of clinical behavior and are characterized by complex chromosomal changes, some of which are associated with distinct clinical phenotypes. We investigated the contribution of genetic variables to staging and histology by logistic regression analyses. We used multiplex ligation-dependent probe amplification (MLPA) to detect segmental genomic imbalances and gene copy number changes in 202 primary NBs. Cases with NB were categorized into four distinct groups based on the genomic changes. Group 1 (48 cases, 23.7%) contained tumors with a 1p deletion and/or MYCN gene amplification (MNA). Group 2 included 46 cases (22.8%) with 3p and/or 11q deletions without 1p deletion and MYCN gene amplification. Tumors harboring at least two commonly observed deletions with or without MNA were classified as Group 3 (25 cases, 12.4%). Tumors with chromosomal imbalance other than MYCN gene amplification and 1p, 3p, and 11q deletions were in Group 4 (83 cases, 41.1%). MYCN gene amplification and 17q gain were significant predisposing factors for unfavorable histology. Significant corr...Continue Reading

References

Aug 1, 1993·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G M BrodeurF Hedborg
Jul 15, 1997·Cancer Genetics and Cytogenetics·B MarshallM G Boavida
Sep 17, 1998·Genes, Chromosomes & Cancer·J VandesompeleF Speleman
Jun 24, 1999·The New England Journal of Medicine·N BownN Van Roy
Jun 20, 2002·Cancer Genetics and Cytogenetics·Lucia GiordaniGian Paolo Tonini
Mar 4, 2003·Nature Reviews. Cancer·Garrett M Brodeur
Mar 21, 2003·Cancer Genetics and Cytogenetics·R L StallingsA O'Meara
Mar 21, 2003·Cancer Genetics and Cytogenetics·Rosa NogueraAntonio Llombart-Bosch
Aug 7, 2003·The Lancet Oncology·Manfred SchwabFrank Berthold
Dec 2, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ruediger SpitzF Berthold
Nov 25, 2005·The New England Journal of Medicine·Edward F AttiyehUNKNOWN Children's Oncology Group
Jul 29, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Shaheen A ChughtaiUNKNOWN UK Children's Cancer Study Group
Jun 26, 2007·Lancet·John M MarisSusan L Cohn
Aug 19, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Judith W M JeukenPieter Wesseling
Jun 25, 2008·Virchows Archiv : an International Journal of Pathology·Eva VillamónRosa Noguera
Sep 3, 2008·Journal of Neuro-oncology·Christian EwaldRolf Kalff
Jan 28, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Isabelle Janoueix-LeroseyOlivier Delattre
Feb 3, 2009·Investigative Ophthalmology & Visual Science·Bertil DamatoSarah E Coupland
Feb 2, 2010·Hematology/oncology Clinics of North America·Julie R ParkHuib Caron
Feb 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Helena CarénTommy Martinsson
Aug 25, 2010·Cancer Science·Miki Ohira, Akira Nakagawara
Oct 27, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bertil DamatoSarah E Coupland
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Inge M AmbrosPeter F Ambros
Nov 1, 2012·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Cornelia Hömig-Hölzel, Suvi Savola
Aug 26, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Navin R PintoSusan L Cohn

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Citations

Jul 10, 2021·Case Reports in Pediatrics·Neofit Spasov, Mariya Spasova

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Methods Mentioned

BETA
biopsy
biopsies
PCR
chromosomal arm aberrations
chromosome arm aberrations
chromosomal aberrations

Software Mentioned

INRG
SPSS
Coffalyzer MLPADAT
GeneMapper

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