The detection of microbial DNA in the blood: a sensitive method for diagnosing bacteremia and/or bacterial translocation in surgical patients

Annals of Surgery
T D KaneJ A Johannigman

Abstract

The purpose was to determine the sensitivity of detecting microbial DNA in the blood of surgical patients as a measure for diagnosing systemic infection and/or translocation from the gut. Microbial infections and translocation of intestinal bacteria are thought to contribute to multiple system organ failure, but bacterial cultures are often negative in patients with this complication. DNA was extracted from the blood of 40 surgical patients and 20 healthy controls. Polymerase chain reaction (PCR) techniques were used to amplify genes from Escherichia coli, Bacteroides fragilis, and a region of 16S ribosomal RNA found in many gram-positive and -negative bacteria. Bacterial DNA genes were not detected in healthy volunteers but were found in all patients with positive blood cultures. All eight transplant patients receiving OKT3 therapy had microbial DNA in their blood, possibly indicating translocation from the gut. Sixty-four percent of critically ill patients had microbial DNA detected in their blood, but only 3 (14%) had positive blood cultures. The PCR method is more sensitive than blood cultures for detecting bacterial components in the blood of critically ill surgical patients and may detect microbial translocation from the ...Continue Reading

References

Jul 30, 1992·The New England Journal of Medicine·D A RelmanS Falkow
Nov 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·O UzunS Unal
Mar 11, 1991·Nucleic Acids Research·M Panaccio, A Lew
Jan 1, 1991·Chest·R L DannerJ E Parillo
Nov 1, 1989·Journal of Clinical Microbiology·C BernetJ Bonnet
Apr 25, 1987·Lancet·J P WaymackJ W Alexander
Oct 1, 1987·Annals of Surgery·J R BorderD Border
Feb 1, 1980·Archives of Surgery·D E FryH C Polk
Mar 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C LumbrerasC V Paya
Feb 1, 1995·Critical Care Medicine·S L Barriere, S F Lowry
Feb 1, 1995·Critical Care Medicine·W J Sibbald, J L Vincent
Mar 1, 1994·Journal of Clinical Microbiology·Y YamashitaM Kaku
Jun 1, 1996·The Journal of Surgical Research·T D KaneC K Ogle
Jan 1, 1996·Clinical Microbiology Reviews·D N Fredricks, D A Relman
Jun 1, 1982·Applied and Environmental Microbiology·J H Paul, B Myers

❮ Previous
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Citations

Dec 10, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·G A Nieuwenhuijzen, R J Goris
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·K T AlscherK R Walley
Feb 8, 2002·World Journal of Gastroenterology : WJG·Wei-Zhong ZhangXue-Rong Chen
Nov 12, 2003·American Journal of Clinical Pathology·M Ali Ansari-LariMichael J Borowitz
May 24, 2006·World Journal of Gastroenterology : WJG·Hans-Olof NilssonTorkel Wadstrom
Mar 22, 2007·Alimentary Pharmacology & Therapeutics·M GattJ MacFie
Apr 24, 2010·American Journal of Respiratory and Critical Care Medicine·Gary W HunninghakeAlix Ashare
May 1, 2015·Critical Care : the Official Journal of the Critical Care Forum·Feng TianJieshou Li
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C A Everson, L A Toth
May 1, 2001·Current Opinion in Critical Care·Gérard Nitenberg
Jan 16, 2002·The Journal of Trauma·James M DotyHarvey J Sugerman
Jan 2, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Maurizio BossolaLuigi Tazza
Jul 28, 2009·Critical Care Medicine·Lutz Eric LehmannFrank Stüber
Aug 8, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhi QiaoJunyou Li
Jul 13, 2011·Pediatric Surgery International·Inaki EizaguirreJose María García Arenzxana
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·E HidakaT Katsuyama
Nov 30, 2000·Current Infectious Disease Reports·L R Baden, B I Eisenstein
May 27, 2004·Clinical Chemistry·Mandira Varma-BasilDavid Alland
Jul 9, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Gerd DöringAlexandra Heininger
Jan 31, 2002·World Journal of Gastroenterology : WJG·W Z ZhangS D Zhang
Jun 5, 2007·Acta Cirúrgica Brasileira·Francisco Edilson Leite PintoHelena Marques Fonseca de Brito
Feb 20, 2010·The British Journal of Surgery·R LemosM Pinho
Feb 6, 2004·Critical Care Medicine·Kevin C DoerschugGary W Hunninghake
Jan 18, 2008·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·N KomenJ F Lange
Sep 10, 2014·Annals of Surgery·Sulaiman R HamarnehRichard A Hodin
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Mariam Klouche, Uwe Schröder
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·María José BordagarayMarcela Hernández
Sep 26, 2013·Indian Journal of Medical Microbiology·C Vaishnavi
Nov 1, 2002·Journal of Clinical Microbiology·Sven KlaschikFrank Stuber
Jan 16, 1999·Journal of Clinical Microbiology·A KlauseggerB Kofler

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