PMID: 9432036Jan 31, 1998Paper

The detection of minimal lymphoma by molecular and combined culture-molecular methods

British Journal of Haematology
G Q WuW Chan

Abstract

Seventy-four patients from a prospective randomized trial comparing autologous bone marrow (ABM) versus blood stem cell (BSC) transplantation after high-dose chemotherapy for intermediate and high grade non-Hodgkin's lymphoma (NHL) were studied for the presence of residual lymphoma prior to transplantation. Pre-transplant bone marrow (BM), peripheral blood (PB) and the ABM or BSC harvest were studied by molecular assays immediately after collection and at weekly intervals after the initiation of in vitro cultures. B-NHLs with t(14:18) at the major breakpoint region (mbr) were monitored by detecting cells with the translocation. Other B-NHLs were monitored with tumour-specific primers and probes to the immunoglobulin heavy chain (IgH) gene complementary determining region (CDR) III. T-NHLs were similarly monitored using the T-cell receptor gamma chain gene V-J junctional region as the tumour-specific marker. Of the 74 patients, seven did not have adequate tumour biopsies for molecular characterization. Of the remaining 67 cases, 35 had identifiable markers for follow-up studies and 20/35 cases (52%) had tumour cells detected in either the pretransplant BM/PB samples or the ABM/BSC harvest. Residual tumours were detected at a hig...Continue Reading

Citations

Aug 31, 2007·Leukemia·S Z PavleticUNKNOWN Center for International Blood and Marrow Transplant Research (CIBMTR), Medical College of Wisconsin, Milwaukee
Feb 15, 2001·Journal of Hematotherapy & Stem Cell Research·J G SharpS S Joshi
Jul 9, 2004·The Tohoku Journal of Experimental Medicine·Atsushi HoshinoTakeshi Sasaki
Oct 26, 2010·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·John F DiPersioStefan Fruehauf
May 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Julie M VoseJames O Armitage

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