PMID: 11325548Apr 28, 2001Paper

The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR

FEMS Microbiology Letters
Michael A HornitzkySteven P Djordjevic

Abstract

The prevalence of complex Shiga toxin-producing Escherichia coli (STEC), i.e. STEC containing accessory virulence factors intimin (eaeA) and/or enterohaemorrhagic E. coli haemolysin (ehxA) and their serotypes were determined in diagnostic bovine faecal samples processed during a 3 months period. The presence of complex STEC was determined using PCR and vancomycin-cefixime-cefsulodin blood agar (BVCCA) using a dual approach which involved (i) direct culture of faecal samples on BVCCA followed by mutiplex PCR of BVCCA positive colonies and (ii) culture of faecal samples enriched in modified EC (mEC) broth (with a complex STEC profile determined by PCR) on BVCCA followed by multiplex PCR of BVCCA positive colonies. Using both techniques complex STEC were isolated from 23 (18.7%) of the 123 faecal samples. Complex STEC were isolated from 14 faecal samples by direct culture on BVCCA and 13 faecal samples yielded complex STEC by culture of mEC broths with a complex STEC profile on BVCCA. Only four samples were positive using both techniques. The serotypes isolated included O5:H-, O26:H-, O26:H11, O91:H21, O111:H-, O111:H8, O104:H11, O113:H21 and O157:H8. This study confirms that non-O157 STEC can be isolated from bovine faeces and th...Continue Reading

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Citations

Nov 27, 2002·Applied and Environmental Microbiology·Michael A HornitzkySteven P Djordjevic
Apr 25, 2007·Critical Reviews in Microbiology·Karl A Bettelheim
Feb 14, 2003·Biotechnology and Bioengineering·Ramaiah Sachidanandham, Karina Yew-Hoong Gin
Nov 20, 2008·Journal of Applied Microbiology·E M MoriartyD R Wood
Oct 31, 2006·Insect Molecular Biology·X QinG M Weinstock
Jun 17, 2014·International Journal of Medical Microbiology : IJMM·Martina BielaszewskaHelge Karch
Nov 8, 2013·Journal of Apicultural Research·Annette Bruun JensenMarla Spivak
Apr 3, 2003·Experimental Biology and Medicine·Karl A Bettelheim

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