The detection of trisomies 8 and 9 in patients with essential thrombocytosis by fluorescence in situ hybridization

Cancer Genetics and Cytogenetics
A ElisM Lishner

Abstract

Essential thrombocytosis (ET) is a clonal, chronic myeloproliferative disorder (MPD) originating from a multipotent stem cell. To date no specific cytogenetic marker has been found in ET. It was recently reported that chromosomal aberrations have been detected by fluorescence in situ hybridization (FISH) in patients with normal karyotypes or nonanalyzable metaphases. Therefore, we evaluated whether trisomies 8 and 9, which are commonly found in MPDs, can be detected in ET by FISH and compared the results with chromosome analysis. Peripheral blood mononuclear cells of patients with essential thrombocytosis were studied by classical chromosome banding and by FISH. We used biotin labeled alpha satellite of chromosome 8 and biotin labeled beta satellite of chromosome 9 as probes for the FISH studies. FISH detected 5 patients with trisomy 8 and 5 with trisomy 9 of the 18 patients evaluated. No trisomy was found by cytogenetic studies. The trisomies were detected by FISH in only a minority of the cells. No correlation was found between the presence of a trisomy and clinical characteristics. FISH is a sensitive method for the detection of trisomies 8 and 9 in patients with ET. The common finding of these chromosomal aberrations in MPD...Continue Reading

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Citations

Oct 31, 2001·Clinical and Laboratory Haematology·I KrsnikP Cabello
Jun 26, 2004·Pathologie-biologie·UNKNOWN Groupe Français de Cytogénétique Hématologique (GFCH)
Jul 12, 2008·Cancer Genetics and Cytogenetics·L Goldberg-BittmanA Amiel
Aug 2, 2006·Archives of Pathology & Laboratory Medicine·Steven Sanchez, April Ewton

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