Abstract
The detection of Plasmodium spp. by the molecular analysis of human feces was reported to be comparable to detection in the blood. We believe that for epidemiological studies using molecular tools, it would be simpler to use feces, which are easier to obtain and require no training for their collection. Our aim was to evaluate the usefulness of feces for the detection of these pathogens towards developing a new tool for their surveillance. Between 2008 and 2010, 451 human fecal samples were collected in two Senegalese villages in which malaria and rickettsioses are endemic. Rickettsia and Plasmodium DNA were detected using quantitative PCR targeting Rickettsia of the spotted fever group, R. felis and Plasmodium spp. Two different sequences were systematically targeted for each pathogen. Twenty of the 451 fecal samples (4.4 %) were positive for Rickettsia spp., including 8 for R. felis. Inhabitants of Dielmo were more affected (18/230, 7.8 %; p = 0.0008) compared to those of Ndiop (2/221, 0.9 %). Children under 15 years of age were more often positive (19/285, 6.7 %) than were older children (1/166, 0.6 %; p = 0.005, odds ratio = 11.79). Only one sample was positive for Plasmodium spp. This prevalence is similar to that found in...Continue Reading
References
Aug 1, 1994·The American Journal of Tropical Medicine and Hygiene·J F TrapeP Ndiaye
Apr 25, 2009·Microbiology·Amel El KhéchineMichel Drancourt
Jun 16, 2009·Emerging Infectious Diseases·Florence FenollarDidier Raoult
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Linda DuvalFrédéric Ariey
Jul 1, 2010·Emerging Infectious Diseases·Allen L RichardsM Kariuki Njenga
Jul 1, 2010·Emerging Infectious Diseases·Cristina SocolovschiDidier Raoult
Jul 28, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Florence FenollarDidier Raoult
Sep 25, 2010·Nature·Weimin LiuBeatrice H Hahn
Dec 30, 2011·PLoS Neglected Tropical Diseases·Alpha Kabinet KeitaFlorence Fenollar
Feb 7, 2012·Emerging Infectious Diseases·Alice N MainaM Kariuki Njenga
Mar 24, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L Duval, F Ariey
Apr 4, 2012·Emerging Infectious Diseases·Milan JirkůJulius Lukeš
Apr 21, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Peeters, E Delaporte
Jul 28, 2012·Journal of Virology·Yingying LiBeatrice H Hahn
Oct 17, 2012·The Journal of Infection·Oleg MediannikovDidier Raoult
Nov 3, 2012·PloS One·Cristina SocolovschiDidier Raoult
Jan 26, 2013·PLoS Neglected Tropical Diseases·Cheikh SokhnaDidier Raoult
Feb 14, 2013·PloS One·Alpha Kabinet KeitaDidier Raoult
Sep 18, 2013·Nature Reviews. Gastroenterology & Hepatology·Sergey R KonstantinovMaikel P Peppelenbosch
Nov 6, 2013·Emerging Infectious Diseases·Oleg MediannikovDidier Raoult
Feb 13, 2014·PloS One·Clémentine RoucherJean-François Trape
May 13, 2014·The Lancet Infectious Diseases·Jean-François TrapeChristophe Rogier
Citations
Jun 25, 2016·Current Tropical Medicine Reports·Lisa D Brown, Kevin R Macaluso
Jul 16, 2016·European Journal of Epidemiology·Miquel Porta, Francisco Bolúmar
Apr 3, 2019·Malaria Journal·Hajri Al-ShehriJ Russell Stothard