PMID: 7544622Aug 23, 1995Paper

The determination of binding constants of micellar-packaged gramicidin A by 13C-and 23Na-NMR

Biochimica Et Biophysica Acta
N JingD W Urry

Abstract

Based on the malonyl gramicidin A structure of a single-stranded head-to-head hydrogen bonded right-handed, beta 6.3-helix in dodecyl phosphocholine (DPC) lipid micelles (Jing et al. (1994) Biophys. J. 66, A353), the determination of cation binding sites for gramicidin A (GA) in DPC micelles becomes a significant step in the study of ion transport through the model channel. First, the investigation of cation binding sites in DPC micellar packaged gramicidin A was achieved by 13C-NMR experiments at 30 degrees C using four C-13 labeled GA samples. Then, the analyses based on two different equations, one for single and one for double occupancy, were employed to evaluate the correct occupancy model for GA in DPC micelles. The results clearly indicate double occupancy to be correct for Na+ ion as well as for K+, Rb+, Cs+, and Tl+ ions. Finally, the binding constants for Na+ ion were also estimated by the measurement of the longitudinal relaxation time (T1) using 23Na-NMR of the same sample at the same ffmperature as used for the 13C-NMR study. The binding constants obtained from 23Na-NMR are essentially equivalent to those determined from the 13C-chemical shifts.

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Citations

May 20, 1998·Biophysical Journal·W NonnerB Eisenberg
Jul 2, 2003·Journal of Biochemical and Biophysical Methods·Maria Carmen BañóConcepción Abad
Oct 14, 2010·The Journal of Physical Chemistry. B·Yuhui LiBenoît Roux
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toby W AllenBenoît Roux
Apr 18, 2006·Biophysical Journal·Anatoly Chernyshev, Samuel Cukierman
Jul 25, 2006·Biophysical Journal·Jacob Devin DurrantDavid D Busath
Nov 5, 1999·Biophysical Journal·B Roux
Apr 23, 2010·Biophysical Journal·Tyson L JonesDavid D Busath
Mar 17, 2007·The Journal of Chemical Physics·Turgut Baştuğ, Serdar Kuyucak
Feb 2, 1999·Journal of Molecular Biology·F Tian, T A Cross
Nov 23, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Gauthier J-P DeblondeGérard Cote
Feb 5, 2021·Journal of Chemical Theory and Computation·Van NgoSergei Noskov
Aug 23, 1995·Biochimica Et Biophysica Acta·N Jing, D W Urry
Jun 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Thamara LaredoJacek Lipkowski

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