The determination of dopamine by a modification of the dihydroxyindole fluorimetric assay

British Journal of Pharmacology
C Atack

Abstract

1. A simplified sensitive fluorimetric assay for dopamine based on the hydroxyindole principle is described. Oxidation of dopamine by ferricyanide and subsequent tautomerization both occur in the same, strongly alkaline, ethanolic solution in the presence of metabisulphite. The reaction is self-regulating, and since times of additions of reagents are relatively unimportant and the final fluorescence is very stable, a large number of samples can be assayed together.2. The method was developed for assaying dopamine in N-ethanolic (50%)-HCl eluates, after purification on a strongly acidic cation exchange column as previously described (Atack & Magnusson, 1970). Portions, up to 1 ml volume, of the 3.5 ml eluate can be taken. No preliminary, time-consuming, neutralization step is required before oxidation. Fluorescence intensity shows a linear relation with concentration for 1-5,000 ng of dopamine per 1.6 ml of final solution, both in pure solutions and in column eluates.3. Fluorescence readings from biological material are given, with evidence for their reproducibility. These, together with other data, demonstrate the relative accuracy of the ;tissue blank' and indicate that amounts of dopamine greater than 3 ng per column can conf...Continue Reading

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Citations

Jan 1, 1980·Psychopharmacology·O JenkinsD M Jackson
Jan 1, 1983·Psychopharmacology·F EdwardsO Jenkins
May 31, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·B Biswas, A Carlsson
Jun 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·D ClarkB S Bunney
Sep 1, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·A DolphinC D Marsden
Feb 25, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·O AlmgrenA Carlsson
Dec 27, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·J A García-SevillaK Folkers
Dec 29, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·W Speckenbach, W Kehr
Jan 1, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·C KelloggJ Jacobson
Jan 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·J A García-SevillaA Carlsson
Mar 29, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·W KehrM Lindqvist
Oct 15, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·W Kehr
Oct 15, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·G Jaim-Etcheverry, L M Zieher
Jan 1, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·M LindqvistA Carlsson
Jul 18, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·N E Andén, M Grabowska
Jan 1, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·W Kehr
Dec 1, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·M C RubioL M Zieher
May 17, 1973·Naunyn-Schmiedeberg's Archives of Pharmacology·S R SniderA Carlsson
May 17, 1973·Naunyn-Schmiedeberg's Archives of Pharmacology·T Magnusson
Aug 24, 1973·Naunyn-Schmiedeberg's Archives of Pharmacology·N E Andén, G Stock
Feb 5, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·S R Snider, B Waldeck
Jan 1, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·A CarlssonW Kehr
Jan 1, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·J DiRaddo, C Kellogg
Jun 21, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·W Kehr
Aug 1, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·C GomesG Trolin
Oct 16, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·W KehrH Herken
May 5, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·N E AndénG Trolin
Aug 15, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·B Biswas, A Carlsson
Aug 15, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·B Biswas, A Carlsson

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