PMID: 11918279Mar 29, 2002Paper

The determination of ketone bodies: preanalytical, analytical and physiological considerations

Clinical and Experimental Medicine
R A LaunK M Schulte

Abstract

The arterial ketone body ratio is calculated as the ratio of arterial levels of acetoacetate/beta-hydroxybutyrate. It correlates with survival in experimental hemorrhagic shock and outcome after liver surgery and myocardial infarction. Procedures for determination of ketone bodies are often laborious and unreliable. As yet the relationship between results from arterial and venous samples is unclear. We therefore describe the determination of the ketone bodies acetoacetate and 3-hydroxybutyrate by an easy, reliable, rapid, inexpensive enzymatic assay using 3-hydroxybutyrate dehydrogenase (E.C. 1.1.1.30) in a semi-automated setting that does not require deproteinization. Preanalytical parameters, including separation from corpuscular elements within 1 h and storage on ice for less than 1 h, must be strictly observed to avoid rapid decay of acetoacetate by spontaneous decarboxylation. The assay has high sensitivity, specificity (+/-5%), and precision (CV <2.5%) with a measurable range of 5-500 micromol/l for either ketone body, and requires only 23.5 microl of plasma. At temperatures below -17 degrees C plasma may be stored for prolonged periods. Results from prospectively scheduled simultaneous sampling of arterial blood and veno...Continue Reading

Citations

Oct 5, 2013·International Journal of Legal Medicine·Terhi KeltanenKatarina Lindroos
Jan 19, 2012·Veterinary Clinical Pathology·Christiane WeingartBarbara Kohn
Jun 25, 2013·Annales d'endocrinologie·Arnaud AginUNKNOWN French Endocrine Society
Mar 1, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Christiane WeingartBarbara Kohn
Feb 6, 2020·BMC Veterinary Research·Nicole S SchmidJean-Michel Hatt

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