The Determination of Parkinson's Drugs in Human Urine by Applying Chemometric Methods

International Journal of Analytical Chemistry
Guzide Pekcan Ertokus

Abstract

The spectrophotometric-chemometric analysis of levodopa and carbidopa that are used for Parkinson's disease was analyzed without any prior reservation. Parkinson's drugs in the urine sample of a healthy person (never used drugs in his life) were analyzed at the same time spectrophotometrically. The chemometric methods used were partial least squares regression (PLS) and principal component regression (PCR). PLS and PCR were successfully applied as chemometric determination of levodopa and carbidopa in human urine samples. A concentration set including binary mixtures of levodopa and carbidopa in 15 different combinations was randomly prepared in acetate buffer (pH 3.5).). UV spectrophotometry is a relatively inexpensive, reliable, and less time-consuming method. Minitab program was used for absorbance and concentration values. The normalization values for each active substance were good (r2>0.9997). Additionally, experimental data were validated statistically. The results of the analyses of the results revealed high recoveries and low standard deviations. Hence, the results encouraged us to apply the method to drug analysis. The proposed methods are highly sensitive and precise, and therefore they were implemented for the deter...Continue Reading

References

Apr 15, 2000·Journal of Pharmaceutical and Biomedical Analysis·K A Sagar, M R Smyth
Oct 10, 2002·Electrophoresis·Pham Thi Thanh HaJos Hoogmartens
Apr 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Morvarid KarimiJoel S Perlmutter
Jul 20, 2007·Journal of Separation Science·Sigrid MennickentMarta de Diego
Dec 26, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chiara MuzziRoberto Guerranti
Dec 29, 2007·Pharmaceutical Development and Technology·Mohammadreza KhanmohammadiKazem Kargosha
Nov 3, 2010·Journal of Pharmaceutical and Biomedical Analysis·F BugamelliM A Raggi
Apr 4, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Venkatraman Junnotula, Hermes Licea-Perez
Jan 9, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Prashant P Raut, Shrikant Y Charde
Jul 27, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Raquel de Oliveira VilhenaRoberto Pontarolo
Feb 26, 2016·Biomedical Chromatography : BMC·Prashant P RautPradeep Bishnoi
Aug 20, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Maha F Abdel-GhanyMenatallah M Youssef
Apr 17, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jingduan ChiFumin Li
May 24, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Venkata Narayana PalakolluSrinivasulu Cherukupalli
Sep 19, 2017·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Amala SoumyanathLynne Shinto

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Methods Mentioned

BETA
PCR

Software Mentioned

Minitab

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