PMID: 4896715Jun 1, 1969Paper

The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation

The Biochemical Journal
U E Loening

Abstract

1. The effects of changes in experimental conditions on the mobility of RNA in polyacrylamide-gel electrophoresis were investigated. 2. The linear relation between log(molecular weight) and electrophoretic mobility was shown to be independent within limits of salt or gel concentration. 3. The relative mobility of RNA with low content of guanylic acid and cytidylic acid residues was decreased in low-ionic-strength buffer. This was related to a small relative decrease in sedimentation coefficient. 4. However, Mg(2+) ion caused almost no increase in mobility although it was associated with large increases in sedimentation coefficient. This suggested opposing actions of Mg(2+) ion on the size and effective charge of the RNA. 5. It is concluded that the method provides a satisfactory measurement of molecular weight, which is almost independent of the nucleotide composition of RNA at moderate salt concentrations.

Citations

May 15, 1979·European Journal of Biochemistry·M R Hartley, C Head
Jan 1, 1977·Journal of Neuroscience Research·A E Ramirez de Guglielmone, B H Duvilanski
Sep 8, 1975·Journal of Molecular Evolution·J GresselN Cohen
Jul 1, 1988·Neurochemical Research·M V RapallinoA Giuditta
Apr 1, 1988·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·I V FilippovichZ A Trebenok
Jul 16, 1973·European Journal of Biochemistry·B Carritt, J M Eisenstadt
Apr 16, 1974·European Journal of Biochemistry·M E HainesR S Palmiter
Jan 21, 1972·European Journal of Biochemistry·L DalgarnoC H Shen
Mar 1, 1980·Journal of Neurochemistry·C J LangfordL Austin
Sep 1, 1981·European Journal of Biochemistry·I BozzoniM Buongiorno-Nardelli
Dec 1, 1979·Current Genetics·H Feldmann, R Großkopf
Jan 1, 1975·Antonie van Leeuwenhoek·A F CroesG Stumm
Apr 1, 1976·European Journal of Biochemistry·D Rosen, M Edelman
Apr 1, 1976·European Journal of Biochemistry·S N Covey, D Grierson
Jan 1, 1979·Archives of Virology·T PetriN J Dimmock
May 15, 1974·European Journal of Biochemistry·D Grierson
Jan 24, 1975·Nature·J M SlackU E Loening
Apr 1, 1971·European Journal of Biochemistry·M EdelmanU Z Littauer
Aug 1, 1992·The Journal of Applied Bacteriology·T WatanabeM Shimizu-Kodota
Sep 1, 1975·FEBS Letters·J TrapmanR J Planta

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