The development and application of a Dot-ELISA assay for diagnosis of southern rice black-streaked dwarf disease in the field.

Viruses
Zhen-Chao WangBao-An Song

Abstract

Outbreaks of the southern rice black-streaked dwarf virus (SRBSDV) have caused significant crop losses in southern China in recent years, especially in 2010. There are no effective, quick and practicable methods for the diagnosis of rice dwarf disease that can be used in the field. Traditional reverse transcription-polymerase chain reaction (RT-PCR) methodology is accurate but requires expensive reagents and instruments, as well as complex procedures that limit its applicability for field tests. To develop a sensitive and reliable assay for routine laboratory diagnosis, a rapid dot enzyme-linked immunosorbent assay (dot-ELISA) method was developed for testing rice plants infected by SRBSDV. Based on anti-SRBSDV rabbit antiserum, this new dot-ELISA was highly reliable, sensitive and specific toward SRBSDV. The accuracy of two blotting media, polyvinylidene fluoride membrane (PVDF membrane) and nitrocellulose filter membrane (NC membrane), was compared. In order to facilitate the on-site diagnosis, three county laboratories were established in Shidian (Yunnan province), Jianghua (Hunan Province) and Libo (Guizhou province). Suspected rice cases from Shidian, Yuanjiang and Malipo in Yunnan province were tested and some determined ...Continue Reading

References

Nov 1, 1984·The American Journal of Tropical Medicine and Hygiene·M G PappasW T Hockmeyer
Jul 15, 1983·Analytical Biochemistry·A EsenS Z Wang
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Sep 30, 2008·Archives of Virology·Heng-Mu ZhangM J Adams

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
dot-ELISA
PCR
electrophoresis

Software Mentioned

BLAST

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.