PMID: 1209688Nov 1, 1975Paper

The development and application of a radioimmunoassay for 18-hydroxy-corticosterone

Steroids
V I MartinF C Bartter

Abstract

A sensitive and specific radioimmunoassay has been developed for 18-hydroxy-corticosterone (18-OH-B) and applied to the measurement of this steroid in peripheral plasma. High specific activity label (3H-18-OH-B) was prepared using the incubation of 3H-corticosterone with duck adrenal mitochondria. Antisera were produced by immunisation with 18-OH-B gamma-lactone 3-oxime conjugated to bovine serum albumin. The antibodies examined showed 100% cross-reactivity with 18-hydroxy-deoxycorticosterone gamma-lactone (18-OH-DOC gamma-lactone), but minimal cross-reactivity with other steroids. Paper chromatography was used to separate 18-OH-DOC gamma-lactone from 18-OH-B gamma-lactone. The interassay precision was 7.6% and the intra-assay precision 11.0%. The accuracy of the method was confirmed by showing a linear relationship between amounts of 18-OH-B added and amounts of 18-OH-B gamma-lactone measured (y = 0.854 X +15.1, r = 0.9. p less than 0.001). The mean plasma level in normal subjects on an ad libitum sodium intake was 225 +/- 92.7 (SD) pg/ml (n = 17) when standing, and 99 +/- 38.3 (SD) pg/ml (n = 6) after lying down for 30 minutes.

References

Dec 1, 1966·The Journal of Clinical Investigation·E G BiglieriN Brust
Mar 1, 1973·The Journal of Clinical Endocrinology and Metabolism·R W FarmerL F Fabre
Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·G E Abraham
Sep 28, 1967·The New England Journal of Medicine·O GoldsmithR Horton
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Aug 1, 1965·The Journal of Clinical Endocrinology and Metabolism·S ULICK, K K VETTER

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Citations

Mar 1, 1978·Journal of Steroid Biochemistry·R Fraser, C P Lantos
Dec 29, 1983·The New England Journal of Medicine·F A WilliamsR M Carey
Jun 1, 1981·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·H Witzgall, S Hassan-Ali
Apr 8, 1988·Journal of Chromatography·O B HollandC Gomez-Sanchez
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·J R Sowers, F W Beck
Dec 1, 1985·The American Journal of Medicine·E ArteagaE G Biglieri
Sep 1, 1988·The American Journal of Medicine·H C TaylorD M Mayes
Sep 1, 1981·The American Journal of Medicine·F ManteroG Faglia
Nov 1, 1982·The American Journal of Physiology·J R Sowers

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