The development and validation of methods for evaluating the immune system in preweaning piglets

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Brandon M ZeiglerBjorn Thorsrud

Abstract

The preweaning piglet has been found to be a valuable research model for testing ingredients used in infant formula. As part of the safety assessment, the neonates' immune system is an important component that has to be evaluated. In this study three concurrent strategies were developed to assess immune system status. The methods included (1) immunophenotying to assess circulating innate immune cell populations, (2) monitoring of circulating cytokines, particularly in response to a positive control agent, and (3) monitoring of localized gastrointestinal tissue cytokines using immunohistochemistry (IHC), particularly in response to a positive control agent. All assays were validated using white papers and regulatory guidance within a GLP environment. To validate the assays precision, accuracy and sample stability were evaluated as needed using a fit for purpose approach. In addition animals were treated with proinflammtory substances to detect a positive versus negative signal. In conclusion, these three methods were confirmed to be robust assays to evaluate the immune system and GIT-specific immune responses of preweaning piglets.

References

Aug 1, 1984·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S Nicklin, K Miller
Sep 15, 2000·Journal of Immunological Methods·M A OwensS B Wormsley
Jun 8, 2001·Veterinary Immunology and Immunopathology·J HernándezE Zenteno
Jun 26, 2001·Toxicologic Pathology·T Holland
Jul 11, 2001·Veterinary Immunology and Immunopathology·K HaversonJ A Ledbetter
Dec 31, 2002·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Zhaokun Ding, Youqing Xu
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R F TsujiB Nowak
Jan 10, 2004·Toxicologic Pathology·James W CrissmanDaryl C Thake
Sep 13, 2005·Domestic Animal Endocrinology·Jeffery A CarrollRandall S Prather
Jan 7, 2006·Pharmaceutical Research·Jean W LeeJohn A Wagner
Mar 2, 2006·Risk Analysis : an Official Publication of the Society for Risk Analysis·Douglas L Weed
May 16, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Josep Bassaganya-Riera, Raquel Hontecillas
Jun 21, 2006·Nature Immunology·Leonore A HerzenbergDavid R Parks
Aug 5, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Holden T Maecker, Joseph Trotter
Jun 15, 2007·Neonatology·Ricki M HelmShanmugam Nagarajan
Jul 31, 2007·Clinics in Laboratory Medicine·Yolanda D Mahnke, Mario Roederer
Jul 8, 2008·Developmental and Comparative Immunology·Wilhelm GernerArmin Saalmüller
May 12, 2009·The Journal of Nutritional Biochemistry·Connie J KimYoshinori Mine
Jul 4, 2009·Cytometry. Part B, Clinical Cytometry·Ruud HulspasD Robert Sutherland
Oct 15, 2009·Pharmaceutical Research·Jo CunliffeRuth Coldwell
May 27, 2010·Nutrition Research Reviews·Paul GuilloteauCornelia C Metges
Jul 27, 2010·Journal of Immunological Methods·Cherie L GreenThomas W Mc Closkey
Oct 16, 2010·Journal of Immunological Methods·Denise M O'HaraVirginia Litwin
Feb 15, 2012·Journal of Visualized Experiments : JoVE·Janice J KimWaliul I Khan
May 16, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kellye S Joiner, Elizabeth A Spangler
Aug 29, 2012·Toxicology Mechanisms and Methods·Edwin G Flamm
Apr 6, 2013·Veterinary Pathology·K N Gibson-CorleyD K Meyerholz
Sep 12, 2013·Cytometry. Part B, Clinical Cytometry·Bruce H DavisDavid Barnett
Sep 12, 2013·Cytometry. Part B, Clinical Cytometry·Brent WoodUNKNOWN ICSH/ICCS Working Group
Dec 18, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Irina Fedorova-DahmsNorman Salem
Jul 16, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Brinda MahadevanHeather E Ferguson
Nov 11, 2014·Annual Review of Animal Biosciences·Jack OdleChad H Stahl
Dec 3, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Paul R Hanlon, Bjorn A Thorsrud
Jan 17, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M L WeinerB Mahadevan

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Jan 17, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M L WeinerB Mahadevan

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