Abstract
The goal of this study was to identify a set of fluorescence in situ hybridization probes for the detection of dysplasia and adenocarcinoma in patients with Barrett's esophagus. We examined 170 brushing specimens from 138 patients with Barrett's esophagus or a history of Barrett's esophagus using fluorescence in situ hybridization with probes to 5p15, 5q21-22, centromere 7, 7p12, 8q24.12-13, centromere 9, 9p21, centromere 17, 17p13.1, 17q11.2-12, 20q13.2, and centromere Y. Receiver-operator curves were used to determine the sensitivity and specificity of various four-probe combinations for detecting low-grade dysplasia, high-grade dysplasia, and esophageal adenocarcinoma. Endoscopic biopsy results were used as the gold standard. Numerous four-probe combinations provided a similarly high sensitivity and specificity. Of these, a set consisting of probes to 8q24, 9p21, 17q11.2, and 20q13.2 was found to have a sensitivity and specificity, respectively, of 70% and 89% for low-grade dysplasia, 84% and 93% for high-grade dysplasia, and 94% and 93% for esophageal adenocarcinoma. This probe set was chosen for future prospective clinical evaluations based on its high sensitivity and specificity, its ability to distinguish adenocarcinoma ...Continue Reading
References
Jul 1, 1996·Gut·A van der BurghM van Blankenstein
Jan 1, 1997·Urological Research·G SauterM J Mihatsch
Jun 1, 1997·Gastroenterology·G W FalkJ E Richter
Dec 3, 1998·Gastroenterology·B KlumpR Porschen
Jul 27, 1999·The Journal of Pathology·H van DekkenC Rosenberg
Jul 13, 2001·American Journal of Clinical Pathology·L BubendorfP Dalquen
Apr 15, 2003·Gut·S H DoakJ M Parry
May 29, 2003·The Journal of Urology·Marek SkacelRaymond R Tubbs
Citations
Jul 31, 2007·Der Pathologe·S Savic, L Bubendorf
May 23, 2009·Current Opinion in Gastroenterology·Yutaka Tomizawa, Kenneth K Wang
Jan 8, 2010·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Edna L MaltbyMarta C Cohen
Aug 29, 2013·Nature Reviews. Gastroenterology & Hepatology·Jamie M J WeaverRebecca C Fitzgerald
May 24, 2014·British Journal of Cancer·C-I GeppertT T Rau
Dec 2, 2014·Gastrointestinal Endoscopy·Andrew Blum, Amitabh Chak
Aug 3, 2014·Gastrointestinal Endoscopy·Margriet R TimmerKenneth K Wang
Mar 31, 2009·Gastroenterology Clinics of North America·Yutaka Tomizawa, Kenneth K Wang
Jul 16, 2010·Journal of Oncology·Agnieszka M RygielKausillia K Krishnadath
May 7, 2015·Gastroenterology Research and Practice·Łukasz SzylbergAndrzej Marszałek
Jan 9, 2015·Current Treatment Options in Gastroenterology·Meghan Jankowski, Sachin Wani
Nov 22, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·William A DayLarry E Morrison
Jan 28, 2010·The American Journal of Gastroenterology·Ganapathy A PrasadKenneth K Wang
Oct 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sanjay Y BangarulingamKeith D Lindor
Mar 27, 2007·Cancer·Agnieszka M RygielKausilia K Krishnadath
Feb 12, 2008·Genes, Chromosomes & Cancer·Agnieszka M RygielKauslillia K Krishnadath
Jun 17, 2008·Cancer·Ganapathy A PrasadKelly Dunagan
Jun 22, 2010·Journal of Cellular Biochemistry·Andrew H FischerPeter Kulesza
Mar 18, 2008·The American Journal of Gastroenterology·Kenneth K WangUNKNOWN Practice Parameters Committee of the American College of Gastroenterology
Mar 5, 2015·Histopathology·Euan A G AllanJames J Going
Jun 6, 2015·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·S M BrankleyK K Wang
May 22, 2013·Current Opinion in Gastroenterology·Daffolyn R Fels Elliott, Rebecca C Fitzgerald
Mar 8, 2017·Digestive Diseases and Sciences·John M PonerosKevin C Halling
Mar 14, 2012·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·K K WangS Krishnadath
Jun 13, 2015·Cancer Control : Journal of the Moffitt Cancer Center·Sherma Zibadi, Domenico Coppola
Apr 1, 2014·Frontline Gastroenterology·Maurice B Loughrey, Brian T Johnston
Oct 15, 2013·Annals of the New York Academy of Sciences·Bas P L WijnhovenValter Nilton Felix
May 2, 2018·Digestive Diseases and Sciences·Fouad Otaki, Prasad G Iyer
Oct 24, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·William M GradyAmitabh Chak
Nov 6, 2020·BMC Gastroenterology·Xiaohong PuJun Chen
Jun 29, 2021·Frontiers in Genetics·Manisha BajpaiHana Aviv