The Development of a Recombinant scFv Monoclonal Antibody Targeting Canine CD20 for Use in Comparative Medicine

PloS One
Saurabh JainTed R Hupp

Abstract

Monoclonal antibodies are leading agents for therapeutic treatment of human diseases, but are limited in use by the paucity of clinically relevant models for validation. Sporadic canine tumours mimic the features of some human equivalents. Developing canine immunotherapeutics can be an approach for modeling human disease responses. Rituximab is a pioneering agent used to treat human hematological malignancies. Biologic mimics that target canine CD20 are just being developed by the biotechnology industry. Towards a comparative canine-human model system, we have developed a novel anti-CD20 monoclonal antibody (NCD1.2) that binds both human and canine CD20. NCD1.2 has a sub-nanomolar Kd as defined by an octet red binding assay. Using FACS, NCD1.2 binds to clinically derived canine cells including B-cells in peripheral blood and in different histotypes of B-cell lymphoma. Immunohistochemical staining of canine tissues indicates that the NCD1.2 binds to membrane localized cells in Diffuse Large B-cell lymphoma, Marginal Zone Lymphoma, and other canine B-cell lymphomas. We cloned the heavy and light chains of NCD1.2 from hybridomas to determine whether active scaffolds can be acquired as future biologics tools. The VH and VL genes fr...Continue Reading

References

Jan 1, 1981·Journal of Immunological Methods·D P Lane, E B Lane
Mar 4, 2000·International Journal of Cancer. Journal International Du Cancer·J BhatiaR H Begent
Aug 13, 2002·Current Pharmaceutical Design·Diane E Milenic
Aug 21, 2002·Journal of Biochemistry, Molecular Biology, and Biophysics : JBMBB : the Official Journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB)·Sheila NathanNoor Embi
Oct 18, 2002·Expert Review of Anticancer Therapy·Antonio J Grillo-López
Jun 24, 2003·Cancer Chemotherapy and Pharmacology·Kensei Tobinai
Aug 3, 2004·Nature Reviews. Drug Discovery·Ismail Kola, John Landis
Jul 12, 2005·Veterinary Pathology·C M JubalaJ F Modiano
Oct 11, 2005·Autoimmunity Reviews·Federico PerosaFranco Dammacco
Nov 16, 2005·Biochemistry·James A ErnstRichard L Vandlen
Sep 12, 2006·Nature Biotechnology·Chand KhannaStephen Withrow
Mar 30, 2007·Nature Clinical Practice. Oncology·Alejandro D Ricart, Anthony W Tolcher
Mar 31, 2007·The Journal of Biological Chemistry·Jiamu DuJianping Ding
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico PerosaFranco Dammacco
Feb 10, 2009·Current Drug Targets·K D Bagshawe
Jan 2, 2010·Journal of Molecular Biology·Efraín SillerJosé M Barral
Mar 9, 2010·PLoS Biology·Abigail L Shearin, Elaine A Ostrander
Apr 8, 2010·The Journal of Allergy and Clinical Immunology·Susan Lee, Mark Ballow
Apr 24, 2010·Nature Reviews. Immunology·Louis M WeinerShangzi Wang
Nov 5, 2010·Annual Review of Genetics·Heidi G ParkerElaine A Ostrander
Nov 9, 2011·Nature Reviews. Clinical Oncology·Jean-Pierre PougetDavid Azria
Feb 3, 2012·Veterinary and Comparative Oncology·O SacornrattanaE A McNiel
Feb 22, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Salomé S PinhoFátima Gärtner
Mar 23, 2012·Nature Reviews. Cancer·Andrew M ScottLloyd J Old
Aug 22, 2012·Methods in Molecular Biology·Glenn A PowersMichael P Wheatcroft
Jan 23, 2013·Nature Reviews. Nephrology·Aditi Sinha, Arvind Bagga
Apr 9, 2013·Expert Opinion on Biological Therapy·Morag Meriel Griffin, Nick Morley
Apr 23, 2013·Expert Opinion on Biological Therapy·Edward M VitalPaul Emery
May 1, 2013·Veterinary Parasitology·Paul R Torgerson
May 2, 2013·Biotechnology and Applied Biochemistry·Juan YangGuanxin Shen
Jun 5, 2013·Cancer Research·Despina Siolas, Gregory J Hannon
Jul 12, 2013·PloS One·Tamara Castillo-TrivinoEmmanuelle Waubant
Oct 5, 2013·Methods in Molecular Biology·Kersten Peldschus, Harald Ittrich
Oct 15, 2013·The Journal of Pathology·Meghna Das ThakurMallika Singh
Oct 18, 2013·The Lancet Oncology·Ramon Luengo-FernandezRichard Sullivan
Oct 26, 2013·Cancer Letters·Prerna MalaneyVrushank Davé
Dec 3, 2013·FEBS Letters·Achim DoernerHarald Kolmar
Jan 11, 2014·Nature Biotechnology·Gunjan Sinha
Mar 5, 2014·Transboundary and Emerging Diseases·P CalistriA Giovannini

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Citations

Jun 1, 2016·The Veterinary Journal·Lisa Y Pang, David J Argyle
Aug 10, 2016·Veterinary and Comparative Oncology·D J Argyle, E Pecceu
Dec 30, 2016·Journal for Immunotherapy of Cancer·Jiwon S ParkRobert J Canter
Oct 20, 2017·Veterinary and Comparative Oncology·E Mazzega, A de Marco
Aug 14, 2018·Frontiers in Veterinary Science·Lorena Bustamante-CórdovaJesús Hernández
Jul 12, 2020·Scientific Reports·Takuya MizunoMasaya Igase
Sep 24, 2019·Antibodies·Qiong WangMichael J Betenbaugh
Feb 22, 2021·Veterinary Immunology and Immunopathology·Paola RoccabiancaMarco Tecilla
Apr 20, 2021·Frontiers in Veterinary Science·Fulvio Riondato, Stefano Comazzi
May 1, 2021·Antibody Therapeutics·Jing ZhangPengfei Zhou
Jun 14, 2021·Journal of Comparative Pathology·Yuki MorinagaTakuya Mizuno
Sep 14, 2021·Frontiers in Veterinary Science·Joana N R DiasFrederico Aires-da-Silva

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Methods Mentioned

BETA
xenograft
xenografts
electrophoresis
biosensors
FACS
PCR
ELISA
transfection
biolayer interferometry
flow cytometry

Software Mentioned

Cell Quest
Attractene
Data Analysis
QUEST

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