The development of animal infection models and antifungal efficacy assays against clinical isolates of Trichosporon asahii, T. asteroides and T. inkin

Virulence
Marçal MarinéGustavo Henrique Goldman

Abstract

The present study developed Galleria mellonella and murine infection models for the study of Trichosporon infections. The utility of the developed animal models was demonstrated through the assessment of virulence and antifungal efficacy for 7 clinical isolates of Trichosporon asahii, T. asteroides and T. inkin. The susceptibility of the Trichosporon isolates to several common antifungal drugs was tested in vitro using the broth microdilution and the E-test methods. The E-test method depicted a lower minimal inhibitory concentration (MIC) for amphotericin and a slightly higher MIC for caspofungin, while MICs observed for the azoles were different but comparable between both methods. All three Trichosporon species established infection in both the G. mellonella and immunosuppressed murine models. Species and strain dependent differences were observed in both the G. mellonella and murine models. T. asahii was demonstrated to be more virulent than the other 2 species in both animal hosts. Significant differences in virulence were observed between strains for T. asteroides in the murine model. In both animal models, fluconazole and voriconazole were able to improve the survival of the animals compared to the untreated control group...Continue Reading

References

Jan 1, 1992·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K J Kwon-ChungJ E Bennett
Feb 1, 1983·The Journal of Infectious Diseases·H M HauptR Saral
Jul 1, 1983·Archives of Dermatology·P M BensonR B Odom
Mar 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T MizobeA Misaki
Jan 1, 1994·Mycoses·E GuéhoB Dupont
May 1, 1993·Mycopathologia·D HospenthalM Kennedy
Aug 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G ChaumentinD Peyramond
May 1, 1996·Mycoses·T ItohY Asada
Feb 19, 1998·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·K YamamotoH Yamaguchi
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum
Nov 14, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·N LussierR Dandavino
Apr 30, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·William J Steinbach, John R Perfect
Sep 27, 2003·Journal of the American Academy of Dermatology·Summer R YoukerJeffrey J Miller
Dec 10, 2003·Journal of Clinical Microbiology·Miguel G MadariagaLaurie Proia
Jan 30, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T J WalshE Anaissie
Feb 24, 2004·Infection and Immunity·Montserrat OrtonedaAntonio Di Pietro
Jul 3, 2004·The Journal of Antimicrobial Chemotherapy·Matteo BassettiDante Bassetti
Sep 17, 2004·Scandinavian Journal of Infectious Diseases·Dimitrios P KontoyiannisIssam I Raad
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Carolina SerenaJosep Guarro
Jun 24, 2005·Infection and Immunity·Eleftherios MylonakisAndrew Diener
Nov 5, 2005·Applied and Environmental Microbiology·Takashi SugitaYoshimasa Uehara
May 26, 2006·Antimicrobial Agents and Chemotherapy·Carolina SerenaJosep Guarro
Sep 29, 2006·Antimicrobial Agents and Chemotherapy·Giovanni Di BonaventuraRaffaele Piccolomini
Nov 14, 2006·Journal of the American Academy of Dermatology·David A KikenNanette B Silverberg
Sep 6, 2007·Virchows Archiv : an International Journal of Pathology·Makoto SanoNorimichi Nemoto
Jan 29, 2008·Mycological Research·Alexandre M FuentefriaPatrícia Valente
Feb 16, 2008·The Journal of Antimicrobial Chemotherapy·Carolina SerenaJosep Guarro
Apr 4, 2009·Medical Mycology·Marcio L RodriguesLeonardo Nimrichter
Jun 6, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sheng-Yuan RuanPo-Ren Hsueh
Jun 23, 2010·Antimicrobial Agents and Chemotherapy·Jesús GuineaEmilio Bouza
Jul 1, 2009·International Journal of Trichology·Anupama S RoshanB Parveen
Dec 24, 2010·Virulence·Beth Burgwyn FuchsEleftherios Mylonakis
Oct 7, 2011·Clinical Microbiology Reviews·Arnaldo L ColomboGuilherme M Chaves
Feb 14, 2012·Methods in Molecular Biology·John FallonKevin Kavanagh
Sep 3, 2013·Biological & Pharmaceutical Bulletin·De-Dong LiYan Wang
Jul 1, 2010·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Agenor Junior Messias SilvestreZoilo Pires de Camargo
Nov 21, 2013·The Journal of Antimicrobial Chemotherapy·Rogelio de J Treviño-RangelGloria M González
Mar 26, 2014·Methods in Molecular Biology·Marios ArvanitisEleftherios Mylonakis
Nov 2, 2014·PloS One·Isabel Antonieta Iturrieta-GonzálezArnaldo Lopes Colombo

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Citations

Nov 21, 2015·Journal of Invertebrate Pathology·Marta WrzosekStanisław Bałazy
Aug 1, 2015·PLoS Pathogens·Marçal MarinéGustavo Henrique Goldman
Jul 1, 2016·Virulence·Olivia L ChampionRichard W Titball
Jul 23, 2016·International Journal of Antimicrobial Agents·Junya de Lacorte SingulaniMaria José Soares Mendes-Giannini
Sep 20, 2018·BMC Infectious Diseases·Eveline Pipolo MilanGuilherme Maranhão Chaves
Aug 14, 2020·Journal of Fungi·Carolina FiracativeWieland Meyer
Feb 8, 2021·Medical Mycology·Rossana de Aguiar CordeiroJosé Júlio Costa Sidrim
Nov 19, 2020·Antibiotics·Jong H KimKirkwood M Land
Apr 17, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E C FranciscoA L Colombo
Oct 1, 2018·Fungal Genetics and Biology : FG & B·Ana Claudia G ZimbresMarcio L Rodrigues
Dec 17, 2021·Future Microbiology·Franz Ag SantosRejane P Neves

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