PMID: 1212561Dec 1, 1975Paper

The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods

British Journal of Pharmacology
K K DigheL Simon

Abstract

1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a 'solid-phase' system. 4 Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.

References

Aug 1, 1972·Prostaglandins·W A Stylos, B Rivetz
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Jul 1, 1972·Prostaglandins·R M ZusmanH R Behrman
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Citations

Mar 19, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·D BenzoniJ Sassard
Jan 1, 1983·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R H HunterN L Poyser
Nov 1, 1982·Prostaglandins, Leukotrienes, and Medicine·F D Lytton, N L Poyser
Jun 1, 1984·Prostaglandins, Leukotrienes, and Medicine·S E Abdel RahimN L Poyser
Nov 1, 1986·Prostaglandins, Leukotrienes, and Medicine·E A Lennon, N L Poyser
Apr 1, 1987·Prostaglandins, Leukotrienes, and Medicine·E A Lennon, N L Poyser
Jul 1, 1987·Prostaglandins, Leukotrienes, and Medicine·N L Poyser, S C Riley
Nov 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K P Bland, N L Poyser
Jul 1, 1977·British Journal of Pharmacology·A N McDougallJ Watson
Feb 1, 1983·Prostaglandins, Leukotrienes, and Medicine·S E Abdel RahimN L Poyser
Mar 1, 1977·British Journal of Pharmacology·K G GreinerJ R Wood
Jun 1, 1978·British Journal of Pharmacology·A Gibson, M Samini

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