The development of early and mature B cells is impaired in mice deficient for the Ets-1 transcription factor

European Journal of Immunology
Stéphanie EyquemJean-Christophe Bories

Abstract

The Ets-1 transcription factor is essential for normal development of the natural killer and T cell lineages; however, its role in B cell development remains poorly understood. To address this issue, we used gene targeting to inactivate Ets-1 in mice (Ets-1(-/-)). We show here that the development of B cell precursors, particularly steps requiring pre-B cell receptor function, is defective in Ets-1(-/-) mice. Peripheral B cell subsets were analyzed in RAG2-deficient mice reconstituted with Ets-1(-/-) fetal liver cells. In such Ets-1(-/-) chimeric mice, B cell precursors develop into IgM/IgD-bearing cells, but B-1a cells as well as transitional-2 and marginal zone B cell subsets of the spleen are absent. In response to B cell receptor stimulation, Ets-1(-/-) splenic B cells fail to express the CD69 and CD25 activation markers. Furthermore, despite activation of ERK and JNK signaling pathways, Ets-1-deficient B cells do not proliferate and die following BCR engagement. These findings demonstrate that the effect of Ets-1 inactivation is not restricted to the terminal B cell differentiation stage, but also affects the development and function of earlier B cell subsets.

References

Mar 6, 1992·Cell·P MombaertsV E Papaioannou
May 1, 1991·The Journal of Experimental Medicine·R R HardyK Hayakawa
Aug 9, 1996·Science·M LeitgesA Tarakhovsky
Dec 1, 1996·Immunology Letters·A Rolink, F Melchers
Jun 17, 2000·Current Opinion in Immunology·K Hayakawa, R R Hardy
Aug 10, 2000·Immunological Reviews·L A Herzenberg
Oct 24, 2000·Immunological Reviews·R GugasyanS Gerondakis
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H H Wortis, R Berland
Feb 15, 2001·Oncogene·J S Yordy, R C Muise-Helmericks
Mar 3, 2001·Current Opinion in Immunology·A G RolinkF Melchers
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J D RichardsA L DeFranco
Mar 13, 2001·Annual Review of Immunology·R R Hardy, K Hayakawa
May 9, 2001·International Reviews of Immunology·F J Staal, H C Clevers
Jan 10, 2002·Genes & Development·Jeffrey J Seidel, Barbara J Graves
May 30, 2002·Nature Reviews. Immunology·Flavius Martin, John F Kearney
Feb 1, 2003·Gene·Tsuneyuki Oikawa, Toshiyuki Yamada
Sep 16, 2003·Molecular Cancer·Jürgen Dittmer
Nov 25, 2003·Immunology·Leo D Wang, Marcus R Clark
Feb 14, 2004·Immunological Reviews·Thomas T SuDavid J Rawlings

❮ Previous
Next ❯

Citations

Sep 22, 2010·The Journal of Experimental Medicine·Enguerran MoulyJean-Christophe Bories
Oct 24, 2009·Nucleic Acids Research·Alexis Vandenbon, Kenta Nakai
May 30, 2012·Arthritis Research & Therapy·Sandra G GuerraDeborah S Cunninghame Graham
Oct 7, 2009·The Review of Diabetic Studies : RDS·Margaret A JordanAlan G Baxter
Jan 5, 2013·Cellular and Molecular Life Sciences : CMLS·Lee Ann Garrett-Sinha
Nov 5, 2014·Leukemia & Lymphoma·Monica TestoniFrancesco Bertoni
Feb 8, 2011·Autoimmunity Reviews·Rui-Xue LengXiang-Pei Li
May 29, 2007·Microbial Pathogenesis·James V RogersCarol L K Sabourin
Aug 2, 2006·Current Opinion in Immunology·Richard R Hardy
Jul 19, 2014·Rheumatic Diseases Clinics of North America·Judith A James
Jul 12, 2017·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Booki Min
Sep 12, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa RussellLee Ann Garrett-Sinha
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·James L ClementsLee Ann Garrett-Sinha
Apr 26, 2017·Frontiers in Immunology·Prontip SaeleeLee Ann Garrett-Sinha
Jul 30, 2005·International Immunology·Duncheng WangLee Ann Garrett-Sinha
Jun 15, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei LuoLee Ann Garrett-Sinha
Jul 26, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica MayeuxAnne B Satterthwaite
Sep 8, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert R HainesJeremy M Boss
Sep 15, 2020·Blood Cancer Discovery·Anna C McCarterMark Y Chiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.