PMID: 9545572May 16, 1998Paper

The development of IL-2 conjugated liposomes for therapeutic purposes

Biochimica Et Biophysica Acta
P J KonigsbergL L Richer

Abstract

A unique immunoliposome has been developed as a drug delivery vehicle for immunotherapy. Human recombinant interleukin-2 (IL-2) has been chemically coupled to the external surface of small unilamellar vesicles (SUVs) containing methotrexate as a candidate immunosuppresive agent in order to specifically direct the drug-bearing liposome to activated T-cells expressing the high affinity IL-2 receptor. This drug delivery system is designed to deliver an immunosuppressive agent to those cells that actively participate in disorders such as graft rejection without delivering an effective but potentially toxic drug to all cells of the immune system as well as other healthy tissues. IL-2 was chemically modified with succinimidyl 4-[p-maleidophenyl butyrate](SMPB) while the receptor binding domain on IL-2 was protected by monoclonal anti-IL-2 bound to Protein A-Silica Gel. The antibody recognizes the receptor binding domain of the IL-2 molecule. The IL-2 was derivatized with S-succinimidyl-S-thioacetate (SATA) in order to add an acetyl thioester group to the lipid and create the complex. The derivatized lipid (SATA-PE) was then part of the liposome formulation containing DSPC:cholesterol: SATA-PE at a mole ratio of 1.5:1.0:0.26. SMPB-IL-...Continue Reading

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Citations

Aug 15, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eri KanaokaKoichiro Hirano
Sep 1, 2000·Advanced Drug Delivery Reviews·S P Vyas, V Sihorkar
Oct 27, 2004·Expert Opinion on Biological Therapy·Joseph G AltinChristopher R Parish
May 18, 2001·Journal of Pharmaceutical Sciences·T Lian, R J Ho
Apr 9, 2001·The Journal of Pharmacy and Pharmacology·E KanaokaK Hirano
Oct 11, 2016·ACS Nano·Stefanie U FrickKerstin Steinbrink
Mar 25, 2011·Angewandte Chemie·Yamuna Krishnan, Friedrich C Simmel
Apr 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Payton A Weidenbacher, Peter S Kim
Sep 13, 2020·Cells·Verena K RakerKerstin Steinbrink
Aug 8, 2020·Advanced Drug Delivery Reviews·Yaping JuSarah F Hamm-Alvarez
Feb 15, 2020·International Immunopharmacology·Alexy Orozco ValenciaMarco Antonio Stephano
Nov 16, 1999·Biochimica Et Biophysica Acta·P J KonigsbergU D Staerz
Oct 25, 2001·The Journal of Organic Chemistry·Bidhan C. Roy, Sanku Mallik

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