The development of radioimmunoassays for fibrinogen degradation products: fragments D and E

British Journal of Haematology
Y B GordonT Chard

Abstract

Fibrinogen degradation products, fragment D (FgD) and fragment E (FgE) have been measured in human serum by specific radioimmunoassays. In addition, the appearance of a neoantigenic determinant on FgD, revealed when fibrinogen is degraded by plasmin has been utilized to develop a specific radioimmunoassay for FgD in plasma (FgDneo). The reagents and conditions used in each assay are described in detail. The mean specific activity was 144 muCi/mug for 125I-labelled FgE and 82 muCi/mug for 125I-labelled FgD. Separation of antibody bound and free antigen was achieved using second antibody. The detection limits of the FgE, FgD and FgDneo assays were 0.8, 1.0 and 6.2 ng/ml respectively. The specificity of each assay with respect to fibrinogen and its degradation fragments has been assessed. Fibrinogen and fragment X cross-reacted markedly in both the FgE and FgD assays, whereas the cross-reaction of fibrinogen was abolished in the FgDneo assay, while the cross-reaction of fragment X was 10%, indicating gradual emergence of the neoantigenic site during digestion of fibriogen. The sensitivity, precision, and specificity of the radioimmunoassay systems described have major advantages over the existing procedures for the measurement of ...Continue Reading

References

May 1, 1971·Journal of the Neurological Sciences·O Petre-Quadens, A De Greef
Jan 1, 1968·Folia phoniatrica·G Kittel
Jan 1, 1971·Scandinavian Journal of Infectious Diseases·B ArvastsonS Winblad
Jan 1, 1971·European Journal of Pharmacology·H C Guldberg, O J Broch
Nov 1, 1971·The Journal of Clinical Endocrinology and Metabolism·B Desbuquois, G D Aurbach
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Feb 26, 1962·Biochimica Et Biophysica Acta·M W MOSESSON

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Citations

Feb 3, 1979·British Medical Journal·Z M RuggeriP M Mannucci
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P A Norton, H S Slayter
Aug 1, 1976·Thrombosis Research·D A LaneV V Kakkar
Mar 1, 1981·British Journal of Haematology·D A Lane
Apr 1, 1976·British Journal of Obstetrics and Gynaecology·Y B GordonT Chard
Apr 1, 1977·British Journal of Haematology·Y B GordonT Chard
Oct 1, 1977·CRC Critical Reviews in Clinical Laboratory Sciences·S T Shaw
May 9, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TanakaK Kato

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