PMID: 6992058Feb 1, 1980Paper

The development of Trypanosoma cruzi in macrophages in vitro. Interaction with lysosomes and host cell fate

Parasitology
R Milder, J Kloetzel

Abstract

The interaction between mouse peritoneal macrophages and 'Y' strain Trypanosoma cruzi bloodstream forms was studied at optical and electron microscopical levels. The method of marking lysosomes with Thorotrast, either before or after infection of cell monolayers with parasites, revealed that secondary lysosomes fused with phagosomes shortly after trypanosome interiorization. In spite of this, 24 h later most parasites were no longer in a vacuole but lay free within the host cell cytoplasm, multiplying actively. At this time, and up to shortly before 96 h when parasites escaped to the external milieu, most parasitized cells were not lethally injured, as revealed by the Trypan blue dye-exclusion test. Only when parasites were released into the external medium was this situation reversed and infected macrophages took up the dye.

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Citations

Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·J M Blackwell, J Alexander
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·A T Bijovsky, R V Milder
Mar 15, 1989·Molecular and Biochemical Parasitology·N W Andrews, M B Whitlow
Aug 1, 1988·The Journal of Experimental Medicine·V LeyV Nussenzweig
Jan 1, 1990·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·S R FarbiarzW De Souza
Mar 1, 1991·Immunology Today·B F Hall, K A Joiner
Mar 1, 1995·Trends in Cell Biology·N W Andrews
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·S el-Shoura
Mar 29, 2012·IUBMB Life·Patricia Silvia RomanoMaría Isabel Colombo
Sep 26, 2000·Experimental and Molecular Pathology·F FrankR E Fretes
Feb 22, 2012·FEMS Microbiology Reviews·Maria Cecilia Fernandes, Norma W Andrews
Feb 15, 2014·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Yasser H Al-Faky
Jul 1, 1984·Revista Do Instituto De Medicina Tropical De São Paulo·J K KloetzelE S Umezawa
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Maria Julia Manso Alves, Renato Arruda Mortara
Feb 5, 2005·Anais Da Academia Brasileira De Ciências·Renato A MortaraSolange da Silva
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·T M CarvalhoE S Coimbra
Dec 1, 1984·Parasitology·G C Coles
Feb 24, 2001·Traffic·L D Sibley, N W Andrews
Feb 23, 2020·Journal of Cell Science·Maria Fátima HortaThiago Castro-Gomes
Feb 13, 2001·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·K A McDonoughY Kress

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