The developmental potential of the inner cell mass of blastocysts that were derived from mouse ES cells using nuclear transfer technology

Cell and Tissue Research
T AmanoY Tsunoda

Abstract

The present study examined the causes of the low developmental potential of enucleated oocytes that have received ES cells and consequent postnatal death of the young. The inner cell masses (ICM) of nuclear-transferred blastocysts or diploid blastocysts were injected into tetraploid blastocysts (group B) or nuclear-transferred tetraploid blastocysts (group C), respectively. The developmental potential of these groups was compared with tetraploid blastocysts injected with ICM of diploid blastocysts (group A). The potential of reconstituted blastocysts to develop into live young in group B increased slightly (5%) but was significantly lower than that in group A (45%). The rate of postnatal death of young in group B did not decrease. The implantation rate of reconstituted blastocysts in group C was very low and no live fetuses were obtained. The results of the present study indicate that the inferior potential of both ICM and trophectoderm cells of nuclear-transferred blastocysts underlies the low developmental rate of nuclear-transferred oocytes receiving ES cells and the higher rate of postnatal death of ES cell-derived young.

Citations

Mar 11, 2003·Cloning and Stem Cells·A OguraF Ishino
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Atsuo OguraTeruhiko Wakayama
Dec 4, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Guy S Eakin, Richard R Behringer
Apr 27, 2010·Developmental Biology·S T BalbachM Boiani
Aug 30, 2006·Biochemical and Biophysical Research Communications·Noriko Wakisaka-SaitoFumitoshi Ishino
Mar 31, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·L N MoroD F Salamone

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