The developmental toxicity and apoptosis in zebrafish eyes induced by carbon-ion irradiation

Life Sciences
Rong ZhouYang Liu

Abstract

Heavy ions have become potentially radiotherapeutic tools. However, studies of the effects on development of normal organs were limited. Using a zebrafish model, this study investigated the potential developmental toxicity and cell apoptosis rates in eyes exposed to carbon-ion irradiation. Zebrafish embryos at 12h post-fertilization (hpf) were irradiated using (12)C(6+) ion beams at doses of 2, 4, and 8 Gy. The reactive oxygen species (ROS) concentration was detected using the dichlorofluorescein-diacetate at 24, 48, and 72 hpf. Apoptosis was assessed by acridine orange staining at 24, 48, and 72 hpf and was also detected using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling assay, at 72 hpf. The expression of genes governing apoptosis was examined using real-time polymerase chain reaction at 24 hpf. Eye size was measured at 144 hpf. Ion irradiation with (12)C(6+) induced a significant increase in cell apoptosis at 24, 48 and 72 hpf. However, there was no significant increase in the ROS concentration at 24, 48, and 72 hpf. The proapoptotic genes, including P53, Bax, and Puma, were significantly upregulated. Two antiapoptotic genes, Mdm2 and Bcl-2, were significantly downregulated, and the express...Continue Reading

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Citations

Aug 16, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jelena HrubikNebojsa Andric
May 8, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Sirui LiBin Liu
Nov 13, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Lu GanHong Zhang
May 13, 2020·Journal of Cellular Physiology·Yang LiuBin Liu
Dec 8, 2019·Ecotoxicology and Environmental Safety·Yang LiuHong Zhang

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