Abstract
Fluid transport in Drosophila melanogaster tubules is regulated by guanosine 3',5'-cyclic monophosphate (cGMP) signalling. Here we compare the functional effects on tubules of different alleles of the dg2 (foraging or for) gene encoding a cGMP-dependent protein kinase (cGK), and show that the fors allele confers an epithelial phenotype. This manifests itself as hypersensitivity of epithelial fluid transport to the nitridergic neuropeptide, capa-1, which acts through nitric oxide and cGMP. However, there was no significant difference in tubule cGK activity between fors and forR adults. Nonetheless, fors tubules contained higher levels of cGMP-specific phosphodiesterase (cG-PDE) activity compared to forR. This increase in cGMP-PDE activity sufficed to decrease cGMP content in fors tubules compared to forR. Challenge of tubules with capa-1 increases cGMP content in both fors and forR tubules, although the increase from resting cGMP levels is greater in fors tubules. Capa-1 stimulation of tubules reveals a potent inhibition of cG-PDE in both lines, although this is greater in fors; and is sufficient to explain the hypersensitive transport phenotype observed. Thus, polymorphisms at the dg2 locus do indeed confer a cGMP-dependent tra...Continue Reading
References
Jan 1, 1992·Genetica·M B Sokolowski, K P Hansell
Sep 1, 1989·Genetics·J S de BelleM B Sokolowski
Nov 3, 1995·The Journal of Biological Chemistry·P J FrenchH R de Jonge
May 1, 1994·The American Journal of Physiology·J A DowK Kaiser
Feb 1, 1993·Genome Génome / Conseil National De Recherches Canada·J S de BelleA J Hilliker
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H S Pereira, M B Sokolowski
Dec 1, 1995·The American Journal of Physiology·S A DaviesN J Tublitz
Aug 1, 1996·The Journal of Clinical Investigation·S GambaryanS M Lohmann
Apr 12, 1996·Molecular and Cellular Biochemistry·A B Vaandrager, H R de Jonge
Sep 20, 1996·The Journal of Biological Chemistry·J L FosterF R Jackson
Dec 20, 1996·Science·A PfeiferR Fässler
Aug 8, 1997·Science·K A OsborneM B Sokolowski
Aug 1, 1997·The American Journal of Physiology·S A DaviesJ A Dow
Jan 20, 1999·Kidney International·T P Dousa
Jan 28, 1999·The Journal of Biological Chemistry·K U HallM D Uhler
May 26, 1999·Journal of Bioenergetics and Biomembranes·J A Dow
Mar 14, 2000·Current Opinion in Cell Biology·A S Edwards, J D Scott
Mar 21, 2000·Nature·J SchlossmannP Ruth
Oct 25, 2000·Insect Biochemistry and Molecular Biology·S Davies
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Laura KeanJulian A T Dow
May 3, 2003·Current Biology : CB·Henrik OsterUrs Albrecht
Jul 5, 2003·Physiological Reviews·Julian T Dow, Shireen A Davies
Jul 11, 2003·American Journal of Physiology. Cell Physiology·Kate E BroderickShireen A Davies
Dec 10, 2003·The Journal of Biological Chemistry·Kate E BroderickShireen A Davies
Citations
Dec 8, 2009·Annual Review of Entomology·Klaus W BeyenbachJulian A T Dow
Sep 4, 2004·Genome Biology·Jing WangJulian A T Dow
Mar 1, 2013·American Journal of Physiology. Renal Physiology·Kristen A RukaEdward M Blumenthal
Feb 7, 2012·Journal of Insect Physiology·Shireen-Anne DaviesSelim Terhzaz
Apr 6, 2013·General and Comparative Endocrinology·Shireen-A DaviesJulian A T Dow
Jun 26, 2004·The Journal of Biological Chemistry·Matthew R MacPhersonShireen-A Davies
Jan 1, 2014·Archives of Insect Biochemistry and Physiology·Ana R CabreraJay D Evans
Sep 6, 2019·Annual Review of Genetics·Ina Anreiter, Marla B Sokolowski
Oct 20, 2005·The Biochemical Journal·Jonathan P DayShireen-A Davies
May 28, 2008·The Biochemical Journal·Jonathan P DayShireen-A Davies
Jan 28, 2005·The Biochemical Journal·Jonathan P DayShireen-A Davies
Feb 8, 2020·Genetics·Erez CohenDonald T Fox
Oct 12, 2018·Journal of Neurogenetics·A M AllenM B Sokolowski
Dec 20, 2013·The Journal of Experimental Biology·Shireen A DaviesJulian A T Dow
Jun 15, 2021·Journal of Neurogenetics·Nawar AlwashJoel D Levine
Nov 2, 2005·Cellular Signalling·Shireen-A Davies
Aug 13, 2021·Journal of Neurogenetics·Aaron M Allen, Marla B Sokolowski