The dg2 (for) gene confers a renal phenotype in Drosophila by modulation of cGMP-specific phosphodiesterase

The Journal of Experimental Biology
Matthew R MacPhersonShireen A Davies

Abstract

Fluid transport in Drosophila melanogaster tubules is regulated by guanosine 3',5'-cyclic monophosphate (cGMP) signalling. Here we compare the functional effects on tubules of different alleles of the dg2 (foraging or for) gene encoding a cGMP-dependent protein kinase (cGK), and show that the fors allele confers an epithelial phenotype. This manifests itself as hypersensitivity of epithelial fluid transport to the nitridergic neuropeptide, capa-1, which acts through nitric oxide and cGMP. However, there was no significant difference in tubule cGK activity between fors and forR adults. Nonetheless, fors tubules contained higher levels of cGMP-specific phosphodiesterase (cG-PDE) activity compared to forR. This increase in cGMP-PDE activity sufficed to decrease cGMP content in fors tubules compared to forR. Challenge of tubules with capa-1 increases cGMP content in both fors and forR tubules, although the increase from resting cGMP levels is greater in fors tubules. Capa-1 stimulation of tubules reveals a potent inhibition of cG-PDE in both lines, although this is greater in fors; and is sufficient to explain the hypersensitive transport phenotype observed. Thus, polymorphisms at the dg2 locus do indeed confer a cGMP-dependent tra...Continue Reading

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Citations

Dec 8, 2009·Annual Review of Entomology·Klaus W BeyenbachJulian A T Dow
Sep 4, 2004·Genome Biology·Jing WangJulian A T Dow
Mar 1, 2013·American Journal of Physiology. Renal Physiology·Kristen A RukaEdward M Blumenthal
Feb 7, 2012·Journal of Insect Physiology·Shireen-Anne DaviesSelim Terhzaz
Apr 6, 2013·General and Comparative Endocrinology·Shireen-A DaviesJulian A T Dow
Sep 6, 2019·Annual Review of Genetics·Ina Anreiter, Marla B Sokolowski
Jan 28, 2005·The Biochemical Journal·Jonathan P DayShireen-A Davies
Oct 12, 2018·Journal of Neurogenetics·A M AllenM B Sokolowski
Dec 20, 2013·The Journal of Experimental Biology·Shireen A DaviesJulian A T Dow
Jun 15, 2021·Journal of Neurogenetics·Nawar AlwashJoel D Levine
Nov 2, 2005·Cellular Signalling·Shireen-A Davies
Aug 13, 2021·Journal of Neurogenetics·Aaron M Allen, Marla B Sokolowski

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