The diaphanous gene of Drosophila interacts antagonistically with multiple wing hairs and plays a key role in wing hair morphogenesis

PloS One
Qiuheng Lu, P N Adler

Abstract

The Drosophila wing is covered by an array of distally pointing hairs that has served as a key model system for studying planar cell polarity (PCP). The adult cuticular hairs are formed in the pupae from cell extensions that contain extensive actin filaments and microtubules. The importance of the actin cytoskeleton for hair growth and morphogenesis is clear from the wide range of phenotypes seen in mutations in well-known actin regulators. Formin proteins promote the formation of long actin filaments of the sort thought to be important for hair growth. We report here that the formin encoding diaphanous (dia) gene plays a key role in hair morphogenesis. Both loss of function mutations and the expression of a constitutively active Dia led to cells forming both morphologically abnormal hairs and multiple hairs. The conserved frizzled (fz)/starry night (stan) PCP pathway functions to restrict hair initiation and activation of the cytoskeleton to the distal most part of wing cells. It also ensures the formation of a single hair per cell. Our data suggest that the localized inhibition of Dia activity may be part of this mechanism. We found the expression of constitutively active Dia greatly expands the region for activation of the c...Continue Reading

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Citations

Mar 21, 2016·Seminars in Cell & Developmental Biology·Danelle Devenport
Feb 6, 2017·Trends in Cell Biology·Paul N Adler, John B Wallingford
Jan 18, 2017·Development·Crystal F Davey, Cecilia B Moens
Sep 22, 2021·Nature Communications·Elena E GrintsevichJonathan R Terman

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Datasets Mentioned

BETA
AAF478454
AAF53922

Methods Mentioned

BETA
GTPases
GTPase
co-IP
dissect
pull downs
pull down
transgenic
confocal microscopy
size exclusion chromatography
immunoprecipitation

Software Mentioned

ImageJ
Metamorph
Excel

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