The Diaphanous-related formin dDia1 is required for highly directional phototaxis and formation of properly sized fruiting bodies in Dictyostelium

European Journal of Cell Biology
Moritz WinterhoffJan Faix

Abstract

Diaphanous-related formins (DRFs) act as downstream effectors of Rho family GTPases and drive the formation and elongation of linear actin filaments in various cellular processes. Here we analyzed the DRF dDia1 from Dictyostelium cells. The biochemical characterization of recombinant dDia1-FH1FH2 by bulk polymerization assays and single filament TIRF microscopy revealed that dDia1 is a rather weak nucleator. Addition of any of the three Dictyostelium profilin isoforms, however, markedly accelerated formin-mediated actin filament barbed end elongation in TIRF assays. Interestingly, filament elongation was significantly faster in presence of DdPFN I (profilin I) when compared to the other two isoforms, suggesting selectivity of dDia1 for DdPFN I. Additionally, we frequently observed dissociation of the formin from growing barbed ends. These findings are consistent with dilution-induced depolymerization assays in presence of dDia1-FH1FH2 showing that dDia1 is a weak capper in comparison with heterodimeric capping protein. To study the physiological role of this formin, we created cell lines lacking dDia1 or overexpressing GFP-tagged dDia1. Of note, constitutively active dDia1 accumulated homogenously in the entire pseudopod sugges...Continue Reading

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Citations

May 23, 2014·Cellular and Molecular Life Sciences : CMLS·Yulia ArtemenkoPeter N Devreotes
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alexander JunemannJan Faix
Mar 23, 2017·Nature Communications·Frieda KageKlemens Rottner
Sep 13, 2017·EMBO Reports·Nina M DrägerThomas R Jahn
Jan 16, 2020·Molecular Biology of the Cell·Mary EckeGünther Gerisch
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christof LitschkoJan Faix

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