The diatom-derived aldehyde decadienal affects life cycle transition in the ascidian Ciona intestinalis through nitric oxide/ERK signalling

Open Biology
Immacolata CastellanoAnna Palumbo

Abstract

Polyunsaturated aldehydes (PUAs) are fatty-acid-derived metabolites produced by some microalgae, including different diatom species. PUAs are mainly produced as a wound-activated defence mechanism against microalgal predators or released from senescent cells at the end of a bloom. PUAs, including 2,4-trans-decadienal (DD), induce deleterious effects on embryonic and larval development of several planktonic and benthic organisms. Here, we report on the effects of DD on larval development and metamorphosis of the ascidian Ciona intestinalis. Ciona larval development is regulated by the cross-talking of different molecular events, including nitric oxide (NO) production, ERK activation and caspase 3-dependent apoptosis. We report that treatment with DD at the competence larval stage results in a delay in metamorphosis. DD affects redox balance by reducing total glutathione and NO levels. By biochemical and quantitative gene expression analysis, we identify the NO-signalling network affected by DD, including the upregulation of ERK phosphatase mkp1 and consequent reduction of ERK phosphorylation, with final changes in the expression of downstream ERK target genes. Overall, these results give new insights into the molecular pathways ...Continue Reading

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Citations

Feb 27, 2016·Scientific Reports·Immacolata CastellanoAnna Palumbo
Nov 28, 2019·Proceedings. Biological Sciences·Marco GerdolImmacolata Castellano
Aug 26, 2020·Development Genes and Evolution·Filomena CaccavaleSalvatore D'Aniello

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR

Software Mentioned

TBLASTX
rimer
REST
ViiATM

Related Concepts

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Apoptosis

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