The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma

Clinical Proteomics
Yang LuJun Dong

Abstract

The various pathogenesis between Clear cell renal carcinoma (CCRCC) and Chromophobe renal carcinoma (CHRCC) contributes to the different tumor growth rate and metastasis. In this study, we explored the distinct proteomic profiles between these two cancers and found different expression of glycogen phosphorylases in two cancers. We explored novel targets by proteomics. Five CCRCC cases and five CHRCC cases were selected for tandem mass tag-labeling liquid chromatography-mass spectroscopy (LC-MS). Gene ontology and KEGG pathway were applied for bioinformatic analysis. Glycogen phosphorylases were detected by Western blotting. CHRCC were younger, more commonly female, and had larger tumors compared to those with CCRCC. 101 differentially expressed proteins (DEPs) in CCRCC and 235 DEPs in CHRCC were detected by LC-MS. It was found that disruption of metabolic pathways, epithelial cell differentiation, and cell response were the common characters for two tumor types. Activation of cell-cell adhesion and oxidation-reduction process stimulate CCRCC growth and epithelial cell differentiation and transferrin transport was involved in CHRCC growth, We also found that oxidative phosphorylation is activated in CHRCC and inhibited in CCRCC....Continue Reading

References

Nov 20, 2009·BMC Clinical Pathology·Ariel A WilliamsJames D Brooks
Dec 12, 2012·Cancer Research·Caitlyn W BarrettChristopher S Williams
Dec 15, 2012·Histopathology·Chung-Chieh WangYung-Ming Jeng
Feb 12, 2013·Redox Report : Communications in Free Radical Research·Ana Savic-RadojevicMarija Matic
Mar 2, 2013·Oncotarget·Elena Favaro, Adrian L Harris
Jul 22, 2014·Nature·Bo LiM Celeste Simon
Oct 31, 2014·Archives of Pathology & Laboratory Medicine·Oleksandr N KryvenkoJonathan I Epstein
Jan 15, 2016·Cancer Cell·A Ari HakimiJames J Hsieh

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Methods Mentioned

BETA
Protein Assay
electrophoresis
X-ray

Software Mentioned

Xcalibur
Proteome Discoverer
Quantity One

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