The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles

Molecular Human Reproduction
S KõksA Salumets

Abstract

Communication between various ovarian cell types is a prerequisite for folliculogenesis and ovulation. In antral follicles granulosa cells divide into two distinct populations of mural and cumulus granulosa cells (CGC), enveloping the antrum and surrounding the oocyte, respectively. Both cell types, with the mural compartment in excess, contribute to the floating granulosa cell (FGC) population in the follicular fluid. The aim of this study was to compare the transcriptomes of FGC and CGC in stimulated antral follicles obtained from 19 women undergoing IVF-ICSI procedure. FGC were obtained from follicular fluid during the follicle puncture procedure and CGC were acquired after oocyte denudation for micromanipulation. Gene expression analysis was conducted using the genome-wide Affymetrix transcriptome array. The expression profile of the two granulosa cell populations varied significantly. Out of 28 869 analysed transcripts 4480 were differentially expressed (q-value < 10(-4)) and 489 showed > or =2-fold difference in the expression level with 222 genes up-regulated in FGC and 267 in CGC. The transcriptome of FGC showed higher expression of genes involved in immune response, hematological system function and organismal injury, ...Continue Reading

References

Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J D BiggersR P Donahue
Aug 1, 1993·Prostaglandins·W J MurdochL A McPherson
May 15, 1996·European Journal of Biochemistry·R BruzzoneD L Paul
Jan 5, 2000·Physiological Reviews·G D NiswenderE W McIntush
Jun 17, 2000·Biochimica Et Biophysica Acta·U SeedorfJ Roch Nofer
Jun 17, 2000·Trends in Endocrinology and Metabolism : TEM·G F Erickson, S Shimasaki
Nov 9, 2000·The Journal of Steroid Biochemistry and Molecular Biology·S GhersevichP Vihko
Jun 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·D F AlbertiniM J Carabatsos
Nov 20, 2001·American Journal of Reproductive Immunology : AJRI·Y TsujiK Koyama
Nov 26, 2002·Endocrinology·Toshio SekiguchiKaoru Miyamoto
Aug 9, 2003·The Journal of Biological Chemistry·Darryl L RussellJoAnne S Richards
Oct 18, 2003·Endocrinology·Venkataraman Sriraman, JoAnne S Richards
Oct 24, 2003·The Journal of Biological Chemistry·Elisabeth RaschpergerJonas Fuxe
Feb 10, 2004·The Journal of Endocrinology·N GérardA-C Lalmanach
Jul 17, 2004·Molecular Endocrinology·Kari M H DoyleJoAnne S Richards
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kenneth P McNattyMika P E Laitinen
Jun 14, 2005·Biochimica Et Biophysica Acta·Junji OhnishiTakayuki Takahashi
Mar 31, 2006·Human Reproduction·Said AssouSamir Hamamah
Apr 14, 2006·Reproductive Biology and Endocrinology : RB&E·Hamish M Fraser
Jun 15, 2006·Human Reproduction·Sanja VujisicMirjana Kujundzic Tiljak

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Citations

Feb 6, 2013·Journal of Assisted Reproduction and Genetics·Serdar CoskunMehmet S Inan
Oct 2, 2013·Human Reproduction Update·Signe AltmäeAndres Salumets
Feb 11, 2011·Molecular Human Reproduction·Ettie MamanAriel Hourvitz
Oct 19, 2011·Clinical & Developmental Immunology·Kadri Haller-KikkataloRaivo Uibo
Nov 17, 2011·PloS One·Zamalou Gisèle OuandaogoSamir Hamamah
Sep 6, 2014·Journal of Human Reproductive Sciences·Rocío Rivera EgeaAlex C Varghese
Oct 3, 2014·BioMed Research International·Tamadir Al-EdaniSamir Hamamah
May 30, 2013·Molecular Endocrinology·Lane K ChristensonJens Vanselow
Dec 17, 2014·Taiwanese Journal of Obstetrics & Gynecology·Kuan-Hao TsuiPeng-Hui Wang
Jan 11, 2012·American Journal of Reproductive Immunology : AJRI·Kadri Haller-KikkataloRaivo Uibo
Oct 19, 2011·Clinical & Developmental Immunology·Aili SarapikAndres Salumets
Apr 4, 2015·Clinical Proteomics·Alberuni M ZamahKatherine E Williams
Jul 6, 2014·Endocrine·Astrud R TuckTheresa E Hickey
Nov 7, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard J Kordus, Holly A LaVoie
Aug 28, 2015·PloS One·Tanja Burnik PaplerLuca Lovrečić
May 11, 2013·Molecular Endocrinology·Agne Velthut-MeikasAndres Salumets
Jul 28, 2020·Ageing Research Reviews·Hamid Reza NejabatiMohammad Nouri
May 9, 2021·Human Reproduction·Lloyd AtkinsonRoger G Sturmey

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