The Differentiation of Human Endometrial Stem Cells into Neuron-Like Cells on Electrospun PAN-Derived Carbon Nanofibers with Random and Aligned Topographies

Molecular Neurobiology
Esmaeil MirzaeiReza Faridi-Majidi

Abstract

Electrospun carbon nanofibers (CNFs) have great potential for applications in neural tissue regeneration due to their electrical conductivity, biocompatibility, and morphological similarity to natural extracellular matrix. In this study, we cultured human endometrial stem cells (hEnSCs) on electrospun CNFs with random and aligned topographies and demonstrated that hEnSCs could attach, proliferate, and differentiate into neural cells on both random and aligned CNFs. However, the proliferation, differentiation, and morphology of cells were affected by CNF morphology. Under the proliferative condition, hEnSCs showed lower proliferation on aligned CNFs than on random CNFs and on tissue culture plate (TCP) control. When cultured on aligned CNFs in neural induction media, hEnSCs showed significant upregulation of neuronal markers, NF-H and Tuj-1, and downregulation of neural progenitor marker (nestin) compared to that on random CNFs and on TCP. In contrast, hEnSCs showed higher expression of nestin and slight upregulation of oligodendrocyte marker (OLIG-2) on random CNFs compared to that on aligned CNFs and on TCP. SEM imaging revealed that differentiated cells extended along the CNF main axis on aligned CNFs but stretched multidirec...Continue Reading

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Citations

Aug 28, 2018·Journal of Cellular Physiology·Saeid Bagheri-MohammadiMahdi Noureddini
Dec 24, 2018·Journal of Biomedical Materials Research. Part a·Elham HasanzadehJafar Ai
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fábio F F GarrudoFrederico Castelo Ferreira
Jan 29, 2020·Biosensors & Bioelectronics·Parvin Samadi PakchinYadollah Omidi
Mar 23, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ghada G AbdoAhmed A Elzatahry

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