The differentiation of preadipocytes and gene expression related to adipogenesis in ducks (Anas platyrhynchos)

PloS One
Shasha WangGuohong Chen

Abstract

Meat quality is closely related to adipose tissues in ducks, and adipogenesis is controlled by a complex network of transcription factors tightly acting at different stages of differentiation especially in ducks. The aim of this study was to establish the preadipocyte in vitro culture system and understand the biological characteristics of expansion of duck adipocyte tissue at the cellular and molecular level. We isolated pre-adipocytes from the subcutaneous fat of three breeds of duck and differentiated them into mature adipocytes using a mixture of insulin, rosiglitazone, dexamethasone, 3-isobutyl-1-methylxanthine, and oleic acid over 0,2, 4, 6, and 8 days. Successful differentiation was confirmed from the development of lipid droplets and their response to Oil Red O, and increasing numbers of lipid droplets were stained red over time. The expression of key marker genes, including peroxisome proliferator activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα), adipocyte fatty acid binding protein 4 (FABP4), and fatty acid synthetase (FAS), gradually increased during pre-adipocyte differentiation. Furthermore, it was verified by interference experiments that the knockdown of PPARγ directly reduced lipid product...Continue Reading

References

Apr 15, 2000·Nature·P G Kopelman
Jan 10, 2002·Genes & Development·Evan D RosenBruce M Spiegelman
Nov 1, 2003·The Journal of Biological Chemistry·Jiang-Wen ZhangM Daniel Lane
Feb 16, 2005·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S R Farmer
Jun 1, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yusuke MatsubaraYukio Akiba
Dec 20, 2005·Cell·Michael Lehrke, Mitchell A Lazar
Dec 15, 2006·Nature·Evan D Rosen, Bruce M Spiegelman
Aug 24, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hui ShiHui Li
Mar 30, 2011·Molecular and Cellular Biochemistry·Fang WangYan Fu
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Apr 3, 2012·Annual Review of Biochemistry·Qi Qun Tang, M Daniel Lane
Nov 28, 2013·Clinics in Liver Disease·Christine McGownZobair M Younossi
Jan 21, 2014·Cell·Evan D Rosen, Bruce M Spiegelman

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
PCR
transfection

Software Mentioned

SPSS

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.