PMID: 8948368Dec 1, 1996Paper

The difficulty of detecting HTLV-1 proviral sequences in patients with mycosis fungoides

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
B A Pancake, D Zucker-Franklin

Abstract

Although most patients with cutaneous T cell lymphomas, including mycosis fungoides (MF) and its leukemic variant, the Sézary syndrome, are seronegative for antibodies to the human T cell lymphotropic viruses (HTLV-I/II), it has recently been shown that > 95% of such patients harbor proviral DNA sequences related to the region of the HTLV genome that encodes the transregulatory/transforming gene, tax. However, the demonstration of HTLV sequences, even after amplification by polymerase chain reaction (PCR), has not been universally successful, and some investigators continue to question this observation. In an effort to resolve this controversy, we have compared published methodologies that have been less successful with techniques currently used in this laboratory. Major differences were found in (a) the nature of the cells used [freshly isolated versus cultured peripheral blood mononuclear cells (PBMC)] and (b) the methods used to prepare samples for PCR (whole cell lysates versus DNA extracts). PBMC from 10 different MF patients and the healthy daughter of 1 of the patients were subjected to comparative analyses. While all of the PBMC lysates were positive, the DNA extract from only one of these individuals revealed HTLV tax ...Continue Reading

References

Jan 23, 1991·JAMA : the Journal of the American Medical Association·H M BauerM M Manos
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Zucker-FranklinD C Zouzias
Oct 1, 1986·Journal of the American Academy of Dermatology·G L WantzinR C Gallo
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D Zucker-FranklinF Quagliata
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B J PoieszR C Gallo
Mar 5, 1993·Science·S J Whittaker, L Luzzatto
Mar 5, 1993·Science·A BazarbachiH de Thé

❮ Previous
Next ❯

Citations

Mar 31, 1998·Journal of the American Academy of Dermatology·P R Carrington
Dec 1, 1996·Trends in Microbiology·Z Kiss-László, T Hohn
Jul 10, 2003·Blood Cells, Molecules & Diseases·Dorothea Zucker-FranklinVesna Najfeld
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·D Zucker-FranklinP M Legler
Dec 21, 2000·Current Rheumatology Reports·D Zucker-Franklin
Jun 1, 1997·Arquivos de neuro-psiquiatria·V FreitasA Melo
Mar 7, 2000·Clinical and Diagnostic Laboratory Immunology·D Zucker-FranklinM Khorshidi
Oct 12, 2001·Annals of the New York Academy of Sciences·D Zucker-Franklin
Feb 18, 2005·Revista Da Sociedade Brasileira De Medicina Tropical·Vandack NobreUNKNOWN Grupo Interdisciplinar de Pesquisas em HTLV-1/2

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.