The direct antiglobulin test: a critical step in the evaluation of hemolysis

American Journal of Hematology
Nicole D ZantekClaudia S Cohn

Abstract

The direct antiglobulin test (DAT) is a laboratory test that detects immunoglobulin and/or complement on the surface of red blood cells. The utility of the DAT is to sort hemolysis into an immune or nonimmune etiology. As with all tests, DAT results must be viewed in light of clinical and other laboratory data. This review highlights the most common clinical situations where the DAT can help classify causes of hemolysis, including autoimmune hemolytic anemia, transfusion-related hemolysis, hemolytic disease of the fetus/newborn, drug-induced hemolytic anemia, passenger lymphocyte syndrome, and DAT-negative hemolytic anemia. In addition, the pitfalls and limitations of the test are addressed. False reactions may occur with improper technique, including improper washing, centrifugation, and specimen agitation at the time of result interpretation. Patient factors, such as spontaneous red blood cell agglutination, may also contribute to false results.

References

Sep 1, 1985·Scandinavian Journal of Haematology·D BarefordL A Tovey
Apr 1, 1987·Transfusion Medicine Reviews·J Freedman
Jan 1, 1982·Acta Haematologica·R J SokolB K Stamps
Feb 1, 1980·Vox Sanguinis·D W GorstF Stratton
Apr 1, 1958·American Journal of Clinical Pathology·C J FLANAGAN, T F MITOMA
Jul 26, 2005·Seminars in Hematology·George Garratty
Sep 28, 2007·Current Opinion in Hematology·Mark H Yazer, Darrell J Triulzi
Oct 2, 2007·Blood Reviews·Lawrence D Petz
Feb 26, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J ShorttM F Cole-Sinclair
May 5, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Susan T Johnson
Dec 17, 2009·Hematology·George Garratty
Mar 4, 2011·Pediatric Blood & Cancer·Patrick T McGannRussell E Ware
Jun 7, 2011·Seminars in Perinatology·Sharon Markham Geaghan

❮ Previous
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Citations

May 22, 2013·Archivum Immunologiae Et Therapiae Experimentalis·José Carlos Jaime-PérezDavid Gómez-Almaguer
Apr 22, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Angela BelsitoClaudio Napoli
Mar 7, 2014·Archives of Disease in Childhood·José Manuel VagaceGuillermo Gervasini
May 27, 2015·Blood Reviews·Elisabeth M MeulenbroekSacha S Zeerleder
Nov 18, 2015·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·M BecheurN E H Toumi
Dec 17, 2015·Revista brasileira de hematologia e hemoterapia·Eloy F Ruiz, Miguel A Cervantes
Nov 1, 2015·Archives of Pathology & Laboratory Medicine·Erin Quist, Scott Koepsell
May 28, 2013·Case Reports in Hematology·Aram BarbaryanAibek E Mirrakhimov
Nov 15, 2014·Archives of Disease in Childhood. Education and Practice Edition·Amy KeirJeannie Callum
Jun 9, 2014·La Revue de médecine interne·A BollotteP Sève
Jun 5, 2015·Hematology/oncology Clinics of North America·Rakhi Naik
Jan 15, 2014·Autoimmunity Reviews·Garrett F BassJoseph M Tuscano
Apr 11, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Garrett S BoothBipin N Savani
May 14, 2016·Acta Paediatrica·Brian K LeeStefan Johansson
Jan 31, 2017·Archives of Pathology & Laboratory Medicine·Victoria Parker, Christopher A Tormey
Sep 8, 2018·Journal of Pharmacy Practice·Kathlyn LimEllen Klapper
Dec 7, 2018·Hematology·John G KeltonDonald M Arnold
Jan 24, 2016·Neonatal Network : NN·Cynthia A Mundy, Jatinder Bhatia
Aug 30, 2014·Journal of Pediatric Hematology/oncology·Tina S IpeDavid F Friedman
May 6, 2017·Journal of Clinical Pharmacy and Therapeutics·K Thiessen, S Kraleti
Sep 2, 2018·Der Anaesthesist·P MöhnleG Wittmann
Dec 4, 2016·Hematology·Theodosia A Kalfa
Dec 7, 2018·JAMA : the Journal of the American Medical Association·Corbin EuleSrikanth Nagalla
Dec 6, 2019·Journal of Family Medicine and Primary Care·Shankar RoyVyas Kumar Rathaur
Sep 11, 2019·Veterinary Clinical Pathology·UNKNOWN Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task ForceBalázs Szladovits
Dec 17, 2014·Cancer Control : Journal of the Moffitt Cancer Center·Radhika Dasararaju, Marisa B Marques
Nov 15, 2019·Vox Sanguinis·Denise Menezes BrunettaJosé Huygens Parente Garcia
Sep 29, 2020·Infection and Drug Resistance·Kamal HamedMaziar Assadi Gehr
Oct 1, 2015·Journal of Pediatric Hematology/oncology·Bülent ÖzgönenelMartin H Bluth
Dec 6, 2016·Transfusion·Alyssa ZimanUNKNOWN the Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Jun 11, 2021·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J CaboV Deneys
Aug 27, 2021·Archives of Disease in Childhood. Education and Practice Edition·Jennifer Delun WilliamsMark Peter Tighe
Nov 21, 2021·Clinical Journal of Oncology Nursing·Jessica Shelly, Michelle Kull

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