PMID: 9188802May 22, 1997Paper

The direct cause of photodamage-induced lysosomal destabilization

Biochimica Et Biophysica Acta
G J Zhang, J Yao

Abstract

Whether membrane lipid photoperoxidation is the immediate cause for lysosomal lysis is still unclear. In this study, we investigated the direct causal factor of photoinduced lysosomal destabilization in a K+-containing solution. Methylene blue (MB)-mediated photodamage caused lysosomal membrane lipid peroxidation and loss of membrane fluidity. Compared with unirradiated lysosomes, the photodamaged lysosomes significantly lost enzyme latency in an isotonic K+-containing solution during a 20-min period of incubation. It indicates an increase in lysosomal K+ permeability. The inward K+ permeation of photodamaged lysosomes was further proved by a K+-induced elevation of internal membrane potential. In addition, the photodamaged lysosomes displayed an increased osmotic sensitivity, showing that MB-mediated photodamage promotes lysosomal osmotic fragility. Although these photoinduced alterations occurred, the lysosomes were relatively stable in an isotonic sucrose medium. In contrast, the organelle destabilized in a photodamage-dependent fashion in an isotonic K+-containing solution. The results indicate that membrane lipid peroxidation does not definitely destabilize lysosomes. The direct cause for the lysosomal destabilization is p...Continue Reading

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Citations

Oct 12, 2000·Cell Biology International·L YangY Z Zheng
May 19, 2007·The Journal of Membrane Biology·Jin-Shan HuGuo-Jiang Zhang
May 30, 2006·Journal of Bioenergetics and Biomembranes·Gu ZhangGuo-Jiang Zhang
Sep 20, 2006·Chemistry and Physics of Lipids·Jiong-Wei WangGuo-Jiang Zhang
Jun 1, 2014·Biochemical and Biophysical Research Communications·Incheol SeoWon-Ki Baek
Mar 9, 2002·Photochemistry and Photobiology·Feng-Yi WanGuo-Jiang Zhang
Nov 2, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna NazarovaJean-Claude Maurizot
Nov 22, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anastasija V EfremenkoAlexey V Feofanov
Mar 12, 2005·World Journal of Gastroenterology : WJG·Elzbieta SkrzydlewskaStanislaw Sulkowski
Jul 31, 2002·Archives of Biochemistry and Biophysics·Feng-Yi WanGuo-Jiang Zhang
Jun 8, 2002·Archives of Biochemistry and Biophysics·Feng-Yi Wan, Guo-Jiang Zhang

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