The discovery of a novel macrophage binding site.

Cellular and Molecular Neurobiology
J M Saavedra, Jaroslav Pavel

Abstract

1. During the course of studies directed to determine the transport of Angiotensin II AT(2) receptors in the rat brain, we found that stab wounds to the brain revealed a binding site recognized by the AT(2) receptor ligand CGP42112 but not by Angiotensin II. 2. We localized this novel site to macrophages/microglia associated with physical or chemical injuries of the brain. 3. The non-Angiotensin II site was also highly localized to inflammatory lesions of peripheral arteries. 4. In rodent tissues, high binding expression was limited to the spleen and to circulating monocytes. A high-affinity binding site was also characterized in human monocytes. 5. Lack of affinity for many ligands binding to known macrophage receptors indicated the possibility that the non-Angiotensin II CGP42112 binding corresponds to a novel site.6. CGP42112 enhanced cell attachment to fibronectin and collagen and metalloproteinase-9 secretion from human monocytes incubated in serum-free medium but did not promote cytokine secretion. 7. When added in the presence of lipopolysaccharide, CGP42112 reduced the lipopolysaccharide-stimulated secretion of the pro-inflammatory cytokines TNF-alpha, IL-1, IL-1 beta, and IL-6, and increased protein kinase A. 8. Molecu...Continue Reading

References

Nov 1, 1992·The Journal of Clinical Investigation·M ViswanathanJ M Saavedra
May 1, 1992·Endocrine Reviews·J M Saavedra
Jan 1, 1991·Endocrinology·K Tsutsumi, J M Saavedra
Aug 30, 1989·Biochemical and Biophysical Research Communications·S WhitebreadM de Gasparo
Apr 17, 1995·Brain Research·F M Heemskerk, J M Saavedra
Apr 29, 1994·Biochemical and Biophysical Research Communications·A M de OliveiraJ M Saavedra
Oct 8, 1997·The American Journal of Physiology·G EgidyJ M Saavedra
Feb 25, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jin ZhouJuan M Saavedra
Apr 20, 2005·Regulatory Peptides·Juan M SaavedraMiroslava Macova
Aug 3, 2005·Cellular and Molecular Neurobiology·Juan M Saavedra
Jul 24, 1940·The Journal of Physiology·E Braun-MenendezJ M Muñoz

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Citations

Oct 2, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Fabien ChauveauBertrand Tavitian

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