PMID: 8602350Feb 1, 1996Paper

The discriminator bases G73 in human tRNA(Ser) and A73 in tRNA(Leu) have significantly different roles in the recognition of aminoacyl-tRNA synthetases

Nucleic Acids Research
K Breitschopf, H J Gross

Abstract

The recognition of human tRNA(Leu) or tRNA(Ser) by cognate aminoacyl- tRNA synthetases has distinct requirements. Only one base change (A73-->G) in tRNA(Leu) is required to generate an efficient serine acceptor in vitro, whereas several changes in three structural domains (the acceptor stem, DHU loop and long extra arm) of tRNA(Ser) are necessary in order to produce a leucine acceptor. Hence, the molecular basis for the discrimination between human tRNA(Ser) and tRNA(Leu) by the seryl-tRNA synthetase depends almost exclusively on a highly specific recognition of the discriminator base G73. In order to elucidate the specific role of the functional groups of this base in discrimination, tRNA(Ser) constructs were made which contain the artificial base analogues 2-aminopurine riboside or inosine at the discriminator position 73. Aminoacylation of these constructs by a HeLa S100 extract showed that molecules with 2-aminopurine riboside, but not with inosine, in position 73 could be serylated at low efficiency. However, the 2-aminopurine riboside and the inosine derivatives of tRNA(Ser) were equally efficient competitive inhibitors of serylation, whereas tRNAs(Ser) with any other natural base at position 73 did not competitively inhi...Continue Reading

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Citations

Mar 29, 2000·Biopolymers·P J Beuning, K Musier-Forsyth
Dec 15, 1996·Nucleic Acids Research·K TomitaK Watanabe
Nov 4, 1998·Nucleic Acids Research·R GiegéC Florentz
Nov 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jean Baptiste ArteroMichael Haertlein
Oct 6, 1998·Annual Review of Biochemistry·S Verma, F Eckstein
May 5, 2004·Journal of Molecular Biology·Bénédicte SohmCatherine Florentz
Apr 27, 2013·RNA Biology·Denisa D MateusManuel A S Santos
Feb 1, 1997·Chemistry & Biology·Y M Hou
Sep 15, 2004·The Journal of Biological Chemistry·Dragana KorencicDieter Söll
Oct 17, 2006·The Journal of Biological Chemistry·Renaud GeslainLluís Ribas de Pouplana
May 23, 2019·Nucleic Acids Research·Jia-Yi FanEn-Duo Wang
Dec 22, 1999·The Journal of Biological Chemistry·A E FischerK Musier-Forsyth

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