Abstract
The BamHI-Z-encoded Epstein-Barr virus (EBV) replication activator (ZEBRA) is a key mediator of the switch from latency to productive cycle in EBV virus. Antibodies against ZEBRA are a marker of EBV reactivation and are regularly found among patients with infectious mononucleosis (IM) or nasopharyngeal carcinoma (NPC), but are only rarely found among healthy EBV-seropositive donors. In order to define the serologically reactive epitopes in the ZEBRA protein, we synthesized a set of overlapping peptides and tested them for reactivity with serum samples from EBV-seronegative persons, patients with NPC, IM, chronic fatigue syndrome, lymphoma or from healthy donors. Three major EBV-specific epitopes were found. These epitopes were further defined and optimized using substitution or truncation analogues of the peptides. Reactivity with epitope number 22 was found in 63% of NPC patients' sera, with < 2% of healthy donors' sera being positive. Serological reactivity with epitope number 19 was associated with IM (57% positive, 5% healthy donors positive). Serum antibodies against epitope 1 were found among healthy donors, but were significantly elevated among patients with NPC, IM or lymphomas. In conclusion, different serologically re...Continue Reading
Citations
Oct 23, 1998·Journal of Medical Virology·S M AaltoK Hedman
Apr 29, 1998·Cancer·S T TsaiR F Ambinder
Mar 1, 1997·Journal of Biomedical Science·C.-H. TsaiC.-S. Yang
Sep 2, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T Lehtinen, M Lehtinen
Jul 8, 2011·Clinical and Vaccine Immunology : CVI·Nina OrlovaFred Wang
Jul 25, 2000·Journal of Virology·J LuC H Tsai
Mar 31, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kelly J YuAllan Hildesheim
Jul 30, 2010·Infectious Agents and Cancer·Amolo S AsitoRosemary Rochford
Dec 14, 1999·Clinical and Experimental Immunology·E DrouetJ L Garnier
Aug 10, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Calypse B AgborsangayaMatti Lehtinen
Apr 22, 2009·Journal of Medical Virology·Erwan PiriouRosemary Rochford
Nov 21, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Rkia DardariMeriem Khyatti
Mar 3, 2007·Clinical and Vaccine Immunology : CVI·Rosamaria TedeschiPaolo De Paoli
May 1, 2009·Clinical and Vaccine Immunology : CVI·Dayana BravoDavid Navarro
May 1, 1996·Clinical and Diagnostic Laboratory Immunology·R TedeschiP De Paoli