The disordered plant dehydrin Lti30 protects the membrane during water-related stress by cross-linking lipids

The Journal of Biological Chemistry
Anjali GuptaThorsten Wohland

Abstract

Dehydrins are intrinsically disordered proteins, generally expressed in plants as a response to embryogenesis and water-related stress. Their suggested functions are in membrane stabilization and cell protection. All dehydrins contain at least one copy of the highly conserved K-segment, proposed to be a membrane-binding motif. The dehydrin Lti30 (Arabidopsis thaliana) is up-regulated during cold and drought stress conditions and comprises six K-segments, each with two adjacent histidines. Lti30 interacts with the membrane electrostatically via pH-dependent protonation of the histidines. In this work, we seek a molecular understanding of the membrane interaction mechanism of Lti30 by determining the diffusion and molecular organization of Lti30 on model membrane systems by imaging total internal reflection- fluorescence correlation spectroscopy (ITIR-FCS) and molecular dynamics (MD) simulations. The dependence of the diffusion coefficient explored by ITIR-FCS together with MD simulations yields insights into Lti30 binding, domain partitioning, and aggregation. The effect of Lti30 on membrane lipid diffusion was studied on fluorescently labeled supported lipid bilayers of different lipid compositions at mechanistically important ...Continue Reading

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Citations

May 15, 2020·Journal of Lipid Research·Jenny Marie AnderssonEmma Sparr
Dec 25, 2019·Archives of Biochemistry and Biophysics·Alejandra A CovarrubiasDavid F Rendón-Luna
Feb 13, 2021·International Journal of Molecular Sciences·Xuechun WangYing Gai

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