The dissociation rate of estrogen receptor alpha from the consensus estrogen response element

Molecular and Cellular Endocrinology
M Szatkowski OzersJ Gorski

Abstract

The rate of dissociation of recombinant, purified human estrogen receptor alpha (ERalpha) from a fluorescein-labeled DNA containing the consensus vitellogenin ERE sequence (F-vitERE) was determined in real time using fluorescence anisotropy. The complex of estradiol-occupied ERalpha with F-vitERE had an apparent dissociation rate of 1.48+/-0.06x10(-2) s(-1) and a half-life of 46.6 s at room temperature. The dissociation rate was characterized by a single exponential decay, suggesting that ER dissociates from the DNA as a preformed dimer, rather than as two individual monomers. The association rate of estradiol-occupied ERalpha for the F-vitERE was calculated as 7x10(6) M(-1) s(-1) based on the dissociation rate measured and previous determinations of the equilibrium dissociation constant (Kd) in similar assay conditions (Ozers et al., 1997). In buffer containing various concentrations of salt, the rate of dissociation of estradiol-occupied ERalpha from F-vitERE was accelerated by increasing salt concentrations. Compared to estradiol-occupied ERalpha, the rate of dissociation of unoccupied ERalpha from the F-vitERE was very similar, indicating that estradiol occupancy does not affect the dissociation rate of ERalpha from the ERE.

References

Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Heyduk, J C Lee
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M SchauerM Beato
Dec 24, 1987·Cell·V KumarP Chambon
Dec 21, 1993·Biochemistry·M FritschJ Gorski
Dec 1, 1996·Endocrine Reviews·M Beato, A Sánchez-Pacheco
Apr 25, 1997·The Journal of Biological Chemistry·B J CheskisC R Lyttle
Dec 31, 1997·The Journal of Biological Chemistry·M S OzersJ Gorski
Feb 4, 1998·The Journal of Steroid Biochemistry and Molecular Biology·C C LandelG L Greene
Feb 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·C M KlingeR Hilf
Aug 26, 1998·Biology of Reproduction·P S CookeG R Cunha
Oct 29, 1998·The Journal of Biological Chemistry·M D DriscollR A Bambara
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A PaigeD M Fowlkes
Jul 16, 1999·Steroids·R Kumar, E B Thompson

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Citations

Jun 6, 2013·International Journal of Endocrinology·Gwenneg KerdivelFarzad Pakdel
Oct 11, 2016·Analytical and Bioanalytical Chemistry·Sandrine BayleDenis Habauzit

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