The distinct function of Tep2 and Tep6 in the immune defense of Drosophila melanogaster against the pathogen Photorhabdus

Virulence
Upasana ShokalIoannis Eleftherianos

Abstract

Previous and recent investigations on the innate immune response of Drosophila have identified certain mechanisms that promote pathogen elimination. However, the function of Thioester-containing proteins (TEPs) in the fly still remains elusive. Recently we have shown the contribution of TEP4 in the antibacterial immune defense of Drosophila against non-pathogenic E. coli, and the pathogens Photorhabdus luminescens and P. asymbiotica. Here we studied the function of Tep genes in both humoral and cellular immunity upon E. coli and Photorhabdus infection. We found that while Tep2 is induced after Photorhabdus and E. coli infection; Tep6 is induced by P. asymbiotica only. Moreover, functional ablation of hemocytes results in significantly low transcript levels of Tep2 and Tep6 in response to Photorhabdus. We show that Tep2 and Tep6 loss-of-function mutants have prolonged survival against P. asymbiotica, Tep6 mutants survive better the infection of P. luminescens, and both tep mutants are resistant to E. coli and Photorhabdus. We also find a distinct pattern of immune signaling pathway induction in E. coli or Photorhabdus infected Tep2 and Tep6 mutants. We further show that Tep2 and Tep6 participate in the activation of hemocytes in...Continue Reading

References

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Citations

Jul 14, 2017·Virulence·Ulrich Theopold, Martin Schmid
Mar 13, 2018·Insect Molecular Biology·I Matetovici, J Van Den Abbeele
Jan 13, 2018·Proceedings. Biological Sciences·Emily L BehrmanPaul S Schmidt
Jul 15, 2017·Frontiers in Immunology·Upasana Shokal, Ioannis Eleftherianos
Feb 22, 2018·Infection and Immunity·Upasana ShokalIoannis Eleftherianos
Dec 7, 2018·Ecology and Evolution·Lumi ViljakainenSylvia Cremer
Jun 15, 2018·Frontiers in Immunology·Anaïs PortetDavid Duval
Feb 13, 2021·Genes·Ioannis Eleftherianos, Christa Heryanto
Jun 23, 2021·Trends in Parasitology·Yaprak Ozakman, Ioannis Eleftherianos

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Methods Mentioned

BETA
PCR
fluorescence microscopy
PCRs

Software Mentioned

Nikon Software Suite
GraphPad Prism7

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