The Distinct Transcriptional Response of the Midgut of Amblyomma sculptum and Amblyomma aureolatum Ticks to Rickettsia rickettsii Correlates to Their Differences in Susceptibility to Infection

Frontiers in Cellular and Infection Microbiology
Larissa A MartinsAndréa C Fogaça

Abstract

Rickettsia rickettsii is a tick-borne obligate intracellular bacterium that causes Rocky Mountain Spotted Fever (RMSF). In Brazil, two species of ticks in the genus Amblyomma, A. sculptum and A. aureolatum, are incriminated as vectors of this bacterium. Importantly, these two species present remarkable differences in susceptibility to R. rickettsii infection, where A. aureolatum is more susceptible than A. sculptum. In the current study, A. aureolatum and A. sculptum ticks were fed on suitable hosts previously inoculated with R. rickettsii, mimicking a natural infection. As control, ticks were fed on non-infected animals. Both midgut and salivary glands of all positively infected ticks were colonized by R. rickettsii. We did not observe ticks with infection restricted to midgut, suggesting that important factors for controlling rickettsial colonization were produced in this organ. In order to identify such factors, the total RNA extracted from the midgut (MG) was submitted to next generation RNA sequencing (RNA-seq). The majority of the coding sequences (CDSs) of A. sculptum differentially expressed by infection were upregulated, whereas most of modulated CDSs of A. aureolatum were downregulated. The functional categories that ...Continue Reading

References

Jan 1, 1975·Annals of the New York Academy of Sciences·W Burgdorfer, L P Brinton
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 30, 1999·Applied and Environmental Microbiology·M L NiebylskiT G Schwan
Aug 28, 1999·The Journal of Biological Chemistry·A C FogaçaS Daffre
Oct 6, 1999·Genome Research·X Huang, A Madan
Nov 5, 1999·Methods in Molecular Biology·S Rozen, H Skaletsky
Nov 24, 1999·Insect Biochemistry and Molecular Biology·P KopácekI Safarík
Dec 11, 1999·Nucleic Acids Research·J SchultzP Bork
Dec 11, 1999·Nucleic Acids Research·A BatemanE L Sonnhammer
Mar 25, 2000·Insect Biochemistry and Molecular Biology·V KovárL Grubhoffer
Apr 5, 2001·Trends in Immunology·P B Armstrong
Jun 28, 2001·Clinical and Diagnostic Laboratory Immunology·M E EremeevaD J Silverman
Dec 26, 2001·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Feb 14, 2002·Insect Molecular Biology·S SangamnatdejP A Nuttall
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 22, 2002·Trends in Biochemical Sciences·Naohiro Inohara, Gabriel Nuñez
Feb 25, 2003·Journal of Medical Entomology·Yoshiro NakajimaMinoru Yamakawa
Jun 12, 2003·Developmental and Comparative Immunology·Lenka GrunclováPetr Kopácek
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Sep 18, 2004·Insect Molecular Biology·J M C RibeiroC Louis
Feb 5, 2005·Journal of Medical Entomology·Nataliia RudenkoLibor Grubhoffer
Aug 17, 2005·Insect Biochemistry and Molecular Biology·Ivo M B FrancischettiJosé M C Ribeiro
Aug 23, 2005·Thrombosis and Haemostasis·Ivo M B FrancischettiJosé M C Ribeiro
Sep 1, 2005·Experimental & Applied Acarology·Daniel E SonenshineTiffany Benzine
Dec 13, 2005·Medical and Veterinary Entomology·W L HynesD E Sonenshine
Jan 25, 2006·Insect Biochemistry and Molecular Biology·José M C RibeiroStephen K Wikel
Jul 4, 2006·International Journal for Parasitology·Takeshi HattaKozo Fujisaki
Nov 23, 2006·Annals of the New York Academy of Sciences·Adriano Pinter, Marcelo B Labruna
Dec 19, 2006·Insect Biochemistry and Molecular Biology·Francisco J Alarcon-ChaidezStephen K Wikel
Jun 7, 2007·Medical and Veterinary Entomology·S M ToddW L Hynes

❮ Previous
Next ❯

Citations

Dec 7, 2017·Frontiers in Cellular and Infection Microbiology·Eliane EstevesAndréa C Fogaça
Jan 18, 2018·Vector Borne and Zoonotic Diseases·Melissa Judith ChaladaRichard Stewart Bradbury
Dec 29, 2020·Parasite Immunology·Charles Edward Hart, Saravanan Thangamani
Nov 22, 2020·Tropical Medicine and Infectious Disease·Janet L H SperlingKatharine E Magor
Mar 20, 2021·Frontiers in Immunology·Andréa C FogaçaSirlei Daffre
Dec 10, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Larissa Almeida MartinsMichail Kotsyfakis
Jun 25, 2021·Trends in Parasitology·Hanna J Laukaitis, Kevin R Macaluso

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRR4277089
PRJNA344771
SRR4301120
GFAA00000000
GFAC00000000

Methods Mentioned

BETA
PCR
glycosylation
RNA-seq

Software Mentioned

blastn
SignalP
CAP3
Soapdenovo Trans
SMART
Abyss
KOG
blastx
Clustal omega
rpsblast

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.