Apr 2, 2020

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

BioRxiv : the Preprint Server for Biology
A. NemeckovaEva Hribova


Despite the recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains limited. In this study, DNA replication timing and interphase chromosome positioning was analyzed in seven Poaceae species differing in genome size. A multidisciplinary approach combining newly replicated DNA labelling by EdU, nuclei sorting by flow cytometry, three-dimensional immuno-FISH, and confocal microscopy revealed similar replication timing order for telomeres and centromeres as well as for euchromatin and heterochromatin in all seven species. The Rabl configuration of chromosomes that lay parallel to each other and their centromeres and telomeres are localized at opposite nuclear poles, was observed in wheat, oat, rye and barley with large genomes, as well as in Brachypodium with a small genome. On the other hand, chromosomes of rice with a small genome and maize with relatively large genome did not assume proper Rabl configuration. In all species, the interphase chromosome positioning inferred from the location of centromeres and telomeres was stable throughout the interphase. These observations extend earlier studies indicating a more complex relation ...Continue Reading

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Mentioned in this Paper

Computer Software
Malus domestica
Determination Aspects
Choroidal Neovascularization
DNA Copy Number Variations
Chromosomes, Human, Pair 2
Massively-Parallel Sequencing

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