PMID: 11905200Jun 1, 1968Paper

The distribution of DNA among dividing mitochondria of Tetrahymena pyriformis

The Journal of Cell Biology
J A Parsons, R C Rustad

Abstract

A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial labeling. Low radioautograph background suggests that mitochondrial DNA is synthesized at the mitochondria themselves. All mitochondria incorporated thymidine-3H within one population-doubling time. Grain counts also showed that the amount of mitochondrial label was retained for four generations and that this label remained randomly distributed among all mitochondria during this time. The results are not consistent with any theory of de-novo or "microbody" origin of mitochondria, but do support the hypothesis that mitochondria are produced by the growth and division of preexisting mitochondria. The stability of the mitochondrial DNA and its distribution among daughter mitochondria satisfy two prerequisites for a genetic material. The possibility is discussed that some of the genetic information for the mitochondrion is contained in the D...Continue Reading

References

May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·M RabinowitzH H Swift
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E Reich, D J Luck
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J H Sinclair, B J Stevens
Oct 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M M Nass
Jun 1, 1965·The Journal of Cell Biology·J A Parsons
Oct 26, 1965·Biochemical and Biophysical Research Communications·K K TewariH R Mahler
Feb 1, 1966·The Journal of Cell Biology·F A Muckenthaler, A P Mahowald
Apr 6, 1966·Biochemical and Biophysical Research Communications·E Moustacchi, D H Williamson
Mar 22, 1966·Biochemical and Biophysical Research Communications·T E Evans
May 19, 1966·Biochimica Et Biophysica Acta·E F van BruggenA M Kroon
Jul 20, 1966·Biochemical and Biophysical Research Communications·J C MounolouP P Slonimski
Oct 5, 1966·Biochemical and Biophysical Research Communications·E Wintersberger
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·D J LUCK, E REICH
Mar 1, 1965·Experimental Cell Research·M M NASSB A AFZELIUS

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Citations

Jun 1, 1972·Biochemical Genetics·R Flavell
Apr 1, 1972·Developmental Biology·J W Chase, I B Dawid
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·C J AversR M Pauli
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J EngbergV Leick
Jan 1, 1973·The Journal of Cell Biology·D R WolstenholmeP Cochran-Fouts
Mar 1, 1973·The Journal of Cell Biology·B Storrie, G Attardi
Aug 1, 1977·The Journal of Cell Biology·J W PosakonyG Attardi
Dec 20, 1979·Nucleic Acids Research·Y FurukawaM Kohno
Nov 1, 1981·Acta Pathologica Japonica·M Satoh, K Yagawa
Aug 1, 1973·CRC Critical Reviews in Biochemistry·H R Mahler
Jan 15, 1973·European Journal of Biochemistry·P Volpe, T Eremenko
Jan 5, 1974·Journal of Molecular Biology·O C Richards, R S Ryan
Dec 1, 1972·Experimental Cell Research·J R Wells, T W James
Jan 22, 1971·Biochemical and Biophysical Research Communications·D H Williamson, E Moustacchi
Oct 1, 1980·International Journal of Andrology·B Bartoov, J Fisher
Mar 1, 1969·Archives of Biochemistry and Biophysics·M A Hilton, R D Dallam
Feb 22, 2018·Antioxidants·Bernard TandlerJason A Mears
Apr 19, 1977·Biochimica Et Biophysica Acta·T FujisawaK Koike

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