PMID: 6105901Sep 22, 1980Paper

The distribution of glutamine synthetase in subcellular fractions of rat brain

Brain Research
S C DennisJ B Clark

Abstract

The subcellular localization of glutamine synthetase, an enzyme fundamental to the compartmentation of glutamate hypothesis, was investigated using brain tissue of adult rats. The distribution of this enzyme in relation to the distribution of glucose-6-phosphatase, glutamate dehydrogenase and acetycholine esterase was studied using a fractionation scheme which had been previously extensively characterized in terms of intramitochondrial enzyme complements. Glutamine synthetase was found to be predominantly localized at the nerve terminal and a number of results suggested a possibble association with the synaptic membrane. The observations are discussed in relation to the compartmentation of glutamate metabolism. Acetate and ammonia are precursors of the 'small' pool of glutamate from which most of the synthesis of glutamine occurs. Since one population of synaptic mitochondria has previously been shown to be enriched in glutamate dehydrogenase and acetyl CoA synthetase and in view of the current observtions that synaptosomes are probably in association with a large proportion of brain glutamine synthetase, it is tentatively suggested that the synaptic complex represents at least in part the site of the 'small' glutamate pool.

References

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Citations

Feb 1, 1987·Neurochemical Pathology·A J Cooper, J C Lai
Oct 12, 2010·Analytical and Bioanalytical Chemistry·Anna Bodzon-KulakowskaJerzy Silberring
Oct 1, 1981·Brain Research·L J KagenA Labissiere
Jan 1, 1983·Progress in Neurobiology·E Kvamme
Jan 1, 1990·Progress in Neurobiology·M Erecińska, I A Silver
Nov 1, 1996·Hearing Research·M EybalinN Renard
Jul 1, 1991·Journal of Neurochemistry·M YudkoffM Erecińska
Aug 1, 1987·Journal of Neurochemistry·H YamamotoY Iwasaki
Nov 1, 1984·Journal of Neurochemistry·J D Rothstein, B Tabakoff

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