PMID: 6164124Jan 1, 1981Paper

The distribution of lipid in the cell structure: an improved method for the electron microscope

Tissue & Cell
V B Wigglesworth

Abstract

An improved partition method for visualizing lipid consists in fixing tissues in paraformaldehyde-glutaraldehyde followed by osmium tetroxide. Three progressive grades of lipid staining are then obtained: (i) by renewed osmium tetroxide alone, (ii) by partition in myrcene or farnesol solutions followed by renewed osmium, (iii) by saturated thymol in sucrose followed by partition and renewed osmium. No additional metallic stains are used. The thymol treatment in (iii) renders 'masked' lipid accessible to partition--the effect being regulated as required by time and temperature. Thymol used before the first osmium facilitates lipid extraction which provides a complementary test for lipid. The possibilities of the method have been demonstrated on sections of familiar tissues of insect (mainly Rhodnius, Hemiptera) and mammal (mouse). By and large the results support what is known already about the distribution of lipid in cells, but observations on lipid in muscle fibres, in the nucleolus and chromatin, in the cells of the adrenal cortex, in the lung and intestine suggest that the method might prove a source of new information.

References

Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Apr 1, 1971·Physiological Reviews·D M Kaplan, R S Criddle
Apr 1, 1969·Chemistry and Physics of Lipids·R B LeslieA M Scanu
Jan 1, 1969·Biochimica Et Biophysica Acta·T J JenkinsonD Chapman
Jul 25, 1955·The Journal of Biophysical and Biochemical Cytology·A J HODGE
Sep 12, 1957·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·V B WIGGLESWORTH
Oct 22, 1963·Biochimica Et Biophysica Acta·D E GREEN, S FLEISCHER

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Citations

Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·R E TracyK Devaney
Jan 1, 1985·Tissue & Cell·V B Wigglesworth
Jan 1, 1989·Tissue & Cell·J C Wright, B M Luke
Aug 1, 1988·Biological Reviews of the Cambridge Philosophical Society·V B Wigglesworth
Apr 1, 1985·The Journal of Applied Bacteriology·N KolankayaJ W Costerton
Jun 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Xiang-Yang ZhuLilach O Lerman

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