PMID: 6108354Dec 1, 1980Paper

The distribution of Mg2+ ATPase and its loss of specific activity upon gradient centrifugation of isolated chromaffin granules

Journal of Neurochemistry
F Konings, W P De Potter

Abstract

Preparations of purified chromaffin granules were subjected to isopycnic sucrose gradient centrifugation. Determination of Mg2+ ATPase activity and catecholamines showed that the distribution of ATPase almost parallelled the distribution of catecholamines. The distribution of ATPase was slightly shifted to lower densities and was suggested to be caused by the heterogeneity of the chromaffin granules. The results therefore provide evidence that ATPase is associated with chromaffin granules. Determination of the recovery of ATPase activity upon gradient centrifugation revealed losses of enzyme activity which were found to be proportional to the dilutions of the granule preparation subjected to gradient centrifugation.

References

Jan 1, 1977·FEBS Letters·T Flatmark, O C Ingebretsen
Mar 10, 1978·Biochimica Et Biophysica Acta·D Njus, G K Radda
Jun 2, 1979·Biochimica Et Biophysica Acta·O TerlandH Kryvi
Oct 1, 1972·Biochemical Pharmacology·F H Schnideer
Oct 1, 1965·Canadian Journal of Biochemistry·P Hagen, A D'Iorio
Feb 14, 1966·Biochimica Et Biophysica Acta·N KirshnerD L Kamin
Mar 1, 1966·The Journal of Physiology·A D Smith, H Winkler
May 1, 1967·The Biochemical Journal·A D Smith, H Winkler
Dec 3, 1957·The Journal of Physiology·H BLASCHKOP HAGEN

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