The distribution of nuclear DNA from human brain-tumor cells

Journal of Neurosurgery
T HoshinoJ W Gray

Abstract

Flow cytometry (FCM) is a technique that measures the quantity of DNA contained in individual nuclei and records a frequency distribution of the DNA content per nucleus in the sampled cell population. Nuclei from a variety of human brain-tumor types were isolated by means of tissue grinding, purified by centrifugation through 40% sucrose (15 minutes at 4000 rpm), fixed with 10% formalin, stained with acriflavin-Feulgen, and analyzed by FCM. Profiles of DNA distribution in histologically benign tumors, such as meningiomas, pituitary adenomas, neuroblastomas, and low-grade astrocytomas, revealed a large diploid population (2C) with a few nuclei in DNA synthesis, as well as a small premitotic population (G2 cells) that contains a 4C DNA complement. In contrast, malignant gliomas, including glioblastomas, consist of more cells in DNA synthesis; these tumor cells show a highly variable distribution of ploidy consisting not only of diploid, and/or aneuploid, but also of triploid, tetraploid, and possibly octaploid populations. Also, a large variability between different regions of each tumor was always observed. In contrast, metastatic brain tumors, despite the fact that they contain a considerable number of cells undergoing DNA synt...Continue Reading

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Methods Mentioned

BETA
flow cytometry
fluorescein microscopy
biopsy
chromosomal aberrations

Related Concepts

Mixed Oligoastrocytoma
Brain Tumor, Recurrent
Cell Nucleus
DNA, Neoplasm
Papillary Ependymoma
Fluorescence
Glioblastoma Multiforme
Mixed Gliomas
Spinal Meningioma
Neoplasm Metastasis

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