The distribution of nuclear DNA from human brain-tumor cells

Journal of Neurosurgery
T HoshinoJ W Gray


Flow cytometry (FCM) is a technique that measures the quantity of DNA contained in individual nuclei and records a frequency distribution of the DNA content per nucleus in the sampled cell population. Nuclei from a variety of human brain-tumor types were isolated by means of tissue grinding, purified by centrifugation through 40% sucrose (15 minutes at 4000 rpm), fixed with 10% formalin, stained with acriflavin-Feulgen, and analyzed by FCM. Profiles of DNA distribution in histologically benign tumors, such as meningiomas, pituitary adenomas, neuroblastomas, and low-grade astrocytomas, revealed a large diploid population (2C) with a few nuclei in DNA synthesis, as well as a small premitotic population (G2 cells) that contains a 4C DNA complement. In contrast, malignant gliomas, including glioblastomas, consist of more cells in DNA synthesis; these tumor cells show a highly variable distribution of ploidy consisting not only of diploid, and/or aneuploid, but also of triploid, tetraploid, and possibly octaploid populations. Also, a large variability between different regions of each tumor was always observed. In contrast, metastatic brain tumors, despite the fact that they contain a considerable number of cells undergoing DNA synt...Continue Reading


Feb 1, 1973·Journal of the Neurological Sciences·D M Mann, P O Yates
May 1, 1967·Neurology·J M Bicknell
Aug 1, 1970·Neurology·C B WilsonM Barker
Jul 1, 1972·Journal of Neurosurgery·T HoshinoD Fewer
Dec 1, 1970·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Mark
May 3, 1968·Science·C J Herman, L W LAPHAM
Mar 14, 1969·Science·M A Van DillaJ R Coulter
Feb 1, 1965·Journal of Neurosurgery·H A LUBS, J H SALMON


Jan 1, 1983·Archives of Oto-rhino-laryngology·M AnnikoJ Wersäll
Jul 1, 1993·Journal of Neuro-oncology·T NishizakiK Sasaki
Oct 1, 1995·Journal of Neuro-oncology·Y YoshiiT Nose
Jan 1, 1985·Acta neurochirurgica·D K LüdeckeW Schmidt
Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·F GiangasperoP C Burger
Jan 1, 1986·Neurosurgical Review·L Calliauw, G Sieben
Oct 1, 1986·Cancer Genetics and Cytogenetics·J A ReyJ Benítez
Aug 1, 1980·Journal of Neurosurgery·S J Mørk, O D Laerum
Apr 1, 1986·Journal of Neurosurgery·T NagashimaS J DeArmond
Mar 1, 1989·Journal of Neurosurgery·M YasueK D Bauer
Jan 1, 1980·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K KawamotoL G Koss
May 1, 1988·European Journal of Cancer & Clinical Oncology·M DanovaP Paoletti
Feb 1, 1985·European Journal of Cancer & Clinical Oncology·G SiebenH Roels
Jul 1, 1989·Neuropathology and Applied Neurobiology·O JimenezJ E McLaughlin
Mar 1, 1980·Histopathology·J De ReuckH V Eecken
Jun 18, 2004·Pathology, Research and Practice·P HabermehlK Hohenfellner
Nov 20, 2014·Cytometry. Part B, Clinical Cytometry·Yu-Wen LinE-Jian Lee
Jan 1, 1993·British Journal of Neurosurgery·S ChatterjeeR V Jeffreys
Apr 1, 1992·Cancer·J M KrosT H van der Kwast
Sep 1, 1979·Cancer·T Hoshino, C B Wilson
Oct 1, 1991·Cancer·H B NewtonM G Malkin
Aug 1, 1984·Journal of Neurochemistry·P MolnarJ Fenstermacher
Jul 1, 1981·Cytometry·O D Laerum, T Farsund
Nov 1, 1989·Journal of Neuro-oncology·A FranziniG Broggi
Sep 1, 1982·Annals of Neurology·W R Shapiro
Jul 9, 1991·International Journal of Cancer. Journal International Du Cancer·M DanovaA Riccardi
Jan 1, 1980·Acta Neuropathologica·J Lehmann, H Krug
Jan 1, 1979·Acta Neurologica Scandinavica·T ShibasakiJ Kawafuchi
Feb 4, 2005·Journal of Neuro-oncology·Basil F El-RayesJohn F Ensley
Apr 12, 1979·Acta Neuropathologica·K KawamotoL G Koss
May 1, 1997·Neurosurgery·P B Dirks, J T Rutka
Jan 1, 1982·Acta Neuropathologica·H Martin, K Voss
May 1, 1992·Acta Neurologica Scandinavica·T AkimuraH Fudaba
Feb 4, 2021·Methods and Protocols·Evrysthenis VartholomatosGeorgios S Markopoulos

Methods Mentioned

flow cytometry
fluorescein microscopy
chromosomal aberrations

Related Concepts

Mixed Oligoastrocytoma
Brain Tumor, Recurrent
Cell Nucleus
DNA, Neoplasm
Papillary Ependymoma
Glioblastoma Multiforme
Mixed Gliomas
Spinal Meningioma
Neoplasm Metastasis

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